Affinity Biologicals Inc. Why Many Laboratories Prefer Polyclonal Antibodies over Monoclonal Antibodies

For many years now, antibodies have played an important role in studying proteins and producing powerful new technologies. Polyclonal or monoclonal antibodies are obtained when a scientist triggers an animal’s immune system by injecting them with an antigen. Although both polyclonal and monoclonal antibodies are viable options in research studies, many laboratories prefer using polyclonal antibodies due to the fact that they are excellent reagents, which when affinity purified can easily compete with monoclonal antibodies in almost any application.

Affinity Biologicals Inc. explains that polyclonals are usually the antibody of choice for the following reasons:

The Advantages of Using Polyclonal Antibodies vs Monoclonal Antibodies

  1. Cost-Effective & Easy to Use – Compared to the alternative, polyclonals are relatively inexpensive to produce because they require minimal production technology and training. As a result, this means lower laboratory costs for the research facility.
  2. Non-Specific – Although monoclonals are often able to create many antibodies, they are sometimes too specific and can decrease the chances for detection. Polyclonal antibodies, on the other hand, produce a high amount of non-specific antibodies.
  3. Diversity – While monoclonals are able to create identical batches of antibodies, polyclonal antibodies have a wide range of variability in their output.
  4. Multiple Recognition Sites – Unlike monoclonal antibodies, polyclonals are not specified to one epitope on an antigen and contain several unique antibodies of different affinity. Having the ability to target multiple epitopes on a single antigen allows for better results in Immunoprecipitation and Chromatin Immunoprecipitation. Additionally, this also amplifies the signal from the target protein, thereby binding with more than one antibody on multiple epitopes which is incredibly helpful for quantification experiments like flow cytometry.
  5. Diversity – While monoclonals are able to create identical batches of antibodies, polyclonal antibodies have a wide range of variability in their output.

Moreover, many research facilities prefer using polyclonals because they are also highly stable, easy to store, tolerate buffer/pH changed well, and allow for better detection. Currently, Affinity Biologicals Inc. has several polyclonal ELISA kits that can be used in the research quantification of factor VII (F7), factor VIII (F8), factor IX (F9), factor XI (F11) and TAFI antigen levels. In addition they also offer custom polyclonal antibody production with the option of sheep, goat, or antibody purification.

Affinity Biologicals Inc. researchers have dedicated over 20 years of experience to the making, purifying, and labeling of polyclonal antibodies. In addition to being a licensed medical device manufacturer that is recognized by the US Food and Drug Administration, Health Canada, and European regulatory authorities all of Affinity Biologicals’ antibodies have also been produced in a provincially licensed research facility. To learn more about any of Affinity Biological’s products visit or contact them directly at:

Affinity Biologicals Inc.

1395 Sandhill Drive

Ancaster, ON, L9G 4V5