Antibodies to Alpha 1-Antitrypsin

Alpha 1-Antitrypsin, human

Antibody Host Catalogue No. Size Product Insert
purified IgG Goat GA1AT-IG 10 mg
affinity purified IgG Goat GA1AT-AP 0.5 mg
purified IgG Sheep SA1AT-IG 10 mg
affinity purified IgG Sheep SA1AT-AP 0.5 mg
affinity purified peroxidase conj. IgG Sheep SA1AT-APHRP 0.2 mg
Matched Pair Antibodies
– For EIA of Alpha 1-Antitrypsin   A1AT-EIA 5 plates
– For EIA of activated Protein C-α1AT Complex   APCAT-EIA 5 plates

Description of Alpha 1-Antitrypsin

Alpha 1Antitrypsin (α1AT), also known as Alpha 1Proteinase inhibitor (α1PI), is the most abundant protease inhibitor in blood and a member of the SERPIN family of proteinase inhibitors. Serum levels are typically 1.3 mg/ml (25 μM) but α1AT is an acute phase protein and concentrations can rise four-fold during inflammatory episodes or tissue injury. Low levels in circulation have been associated with pulmonary disease such as emphysema. α1AT is a single chain molecule with a mass of 52,000 daltons that is produced primarily in the liver and to a lesser extent by blood monocytes and intestinal epithelium. Based on association rates, the primary target enzyme for α1AT is believed to be neutrophil elastase1,2, but α1AT is a broad-spectrum inhibitor for many serine proteinases and the main role of α1AT in vivo is likely that of a “backup” inhibitor and proteinase scavenger in fluids and tissues. Although the association rates of α1AT with other enzymes are lower, the high concentration in plasma makes it an important inhibitor of activated Protein C, activated FXI, thrombin and plasmin1-4. Enzyme inhibition by α1AT occurs through proteolytic cleavage between Met358 and Ser359, which induces a conformational change in α1AT locking the enzyme into a stable, inactive 1:1 enzyme-inhibitor complex.

References:

1. Johnson D, Travis J; Oxidative Inactivation of Human α-1-Proteinase Inhibitor; JBC 254, pp4022-4026, 1979.

2. Travis J, Johnson D; Human α-1-Proteinase Inhibitor; Methods in Enzymology, 80, pp 754-765, 1981.

3. Heeb MJ, Griffin JH; Physiologic Inhibition of Human Activated Protein C by α-1-Trypsin Inhibitor; JBC 263, pp11613-11616, 1988.

4. Scott CF, Schapira M, James HL, Cohen AB, Colman RW; The Inactivation of Factor XIa by plasma protease inhibitors: Predominant role of α1protease inhibitor and protective effect of high molecular weight kininogen. J Clin Invest 69, pp 844, 1982.

Related Products:

GA1AT-IG Goat anti-human α1AT, whole IgG from antiserum

SA1AT-IG Sheep anti-human α1AT, whole IgG from antiserum

SA1AT-AP Sheep anti-human α1AT, affinity purified IgG

SA1AT-APHRP Sheep anti-human α1AT, APIgG-peroxidase

A1AT-EIA Paired antibody set for ELISA of α1AT , 5 x 96 wells

APCAT-EIA Paired antibody set for ELISA of APC-α1AT complexes