Alpha 2 -Antiplasmin, human
|Antibody||Host||Catalogue No.||Size||Product Insert|
|purified IgG||Goat||GA2AP-IG||5 mg|
|affinity purified IgG||Goat||GA2AP-AP||0.5 mg|
|peroxidase conjugated IgG||Goat||GA2AP-HRP||0.2 mg|
|purified IgG||Sheep||SA2AP-IG||10 mg|
Description of Alpha 2-Antiplasmin
Alpha 2-Antiplasmin (ÃŽÂ±2AP), also known as Alpha 2-Plasmin Inhibitor (ÃŽÂ±2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70 ÃŽÂ¼g/ml (~1 ÃŽÂ¼M). ÃŽÂ±2AP is a single chain molecule with a mass of 67 kDa as determined by SDS-PAGE. The primary target enzyme for ÃŽÂ±2AP is plasmin, but ÃŽÂ±2AP also acts as secondary or Ã¢â‚¬Å“backupÃ¢â‚¬Â inhibitor of activated FXI, activated Protein C and trypsin. Inhibition of these enzymes by ÃŽÂ±2AP occurs through proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme-ÃŽÂ±2AP complex. ÃŽÂ±2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of ÃŽÂ±2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of ÃŽÂ±2AP (~65 kDa) has a reduced rate of plasmin inhibition and has been referred to as the Ã¢â‚¬Å“slow formÃ¢â‚¬Â of ÃŽÂ±2AP. During fibrin formation, a portion of circulating ÃŽÂ±2AP is cross-linked to the ÃŽÂ±-chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin1-4.
1. Aoki N, Suni Y, Miura O, Hirosawa S; Human ÃŽÂ±2Plasmin Inhibitor; Methods in Enzymology, 223, pp 185-197, 1993.
2. Shieh BH, Travis J; The Reactive Site of Human ÃŽÂ±2-Plasmin Inhibitor ; JBC 262, pp 6055-6059, 1987.
3. Moroi M, Aoki N; Isolation and Characterization of ÃŽÂ±2-Plasmin Inhibitor from Human Plasma; JBC 251, pp 5956-5965, 1976.
4. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.
GA2AP-IG Goat anti-human ÃŽÂ±2AP, whole IgG from antiserum
GA2AP-AP Goat anti-human ÃŽÂ±2AP, affinity purified IgG
GA2AP-HRP Goat anti-human ÃŽÂ±2AP, APIgG-peroxidase conjugate
SA2AP-AP Sheep anti-human ÃŽÂ±2AP, affinity purified IgG
A2AP-DP Plasma deficient in Alpha 2-Antiplasmin, immune-depleted, Frozen
A2AP-LDP Plasma deficient in Alpha 2-Antiplasmin, immune-depleted, Lyophilized