Affinity Biologicals, Inc. is conveniently located within the “Golden Horseshoe” area of South Western Ontario, Canada.
Our manufacturing facility is located at:
1348 Sandhill Drive Ancaster, Ontario Canada L9G 4V5
Meet with us at these upcoming Exhibitions:
IMPROVED RECOGNITION OF B-DOMAIN DELETED FVIII IN PLASMA WITH A NEW COMMERCIALLY AVAILABLE
VISULIZE™ F8PLUS ANTIGEN KIT
Ran Ni, Paul Erb, Joanne Della Maestra, Denise Foulon, Karen M. Black, Hugh Hoogendoorn
Affinity Biologicals, Ancaster, Ontario, Canada
Background: The 1st generation of VisuLize Factor VIII Antigen Kit was found to under-recover a recombinant B-domain deleted (BDD) FVIII antigen (Xyntha®) relative to plasma FVIII. As BDD forms of FVIII are becoming the most administered replacement products in Hemophilia A, there is a need for a commercially available FVIII antigen kit to reliably measure these truncated forms in plasma, using a WHO-traceable, plasma-based calibrator.
Methods: The 1st generation ELISA (VisuLize Factor VIII Antigen Kit) was reoptimized to select for conditions that improved the recovery of BDD-FVIII (Xyntha). The resulting 2nd generation of VisuLize Factor VIII Antigen Plus (F8PLUS Antigen) Kit still uses a normal plasma calibrator and plasma controls.
Objective: To provide a research tool for harmonizing the quantification of recombinant full-length and BDD-FVIIIs relative to a plasma-based calibrator.
