Factor FIX – Published Papers Using Affinity Biologicals Products

2013

HTML] Integration Profile and Safety of an Adenovirus Hybrid-Vector Utilizing Hyperactive Sleeping Beauty Transposase for Somatic Integration

W Zhang, M Muck-Hausl, J Wang, C Sun, M Gebbing… – PLOS ONE, 2013
The cFIX enzyme-linked immunosorbent assay (ELISA) measuring cFIX levels in murine serum was performed as described earlier [21]. In brief, for coating of ELISA plates, the polyclonal rabbit anti-cFIX primary antibody (RACIX-IG, Affinity Biologicals Inc.) was diluted 1:1000. … 
 

LONG-ACTING COAGULATION FACTORS AND METHODS OF PRODUCING SAME

UE Fima, G Hart – US Patent 20,130,243,747, 2013
FIG. 1A. Shows a bar graph showing harvests limited, diluted, transfected, and selected cells with FIX-CTP and FIX-CTP-CTP variants in the presence of 5 μg/ml of Vitamin K3. The level of FIX was quantified using Human FIX ELISA kit (Affinity Biologicals; Cat. No. … 
 

Role of antigen-specific regulatory CD4+ CD25+ T cells in tolerance induction after neonatal IP administration of AAV-hF. IX

Y Shi, R Falahati, J Zhang, L Flebbe-Rehwaldt… – Gene Therapy, 2013
standard curve. A polyclonal goat anti-h.FIX antibody conjugated with horseradish peroxidase (HRP) (Affinity Biologicals, Hamilton, Ontario, Canada) was used as the secondary antibody in a dilution of 1:1000. After applying
 

Cell-matrix Interactions of Factor IX (FIX)-engineered human mesenchymal stromal cells encapsulated in RGD-alginate vs. Fibrinogen-alginate microcapsules

B Sayyar, M Dodd, L Marquez-Curtis… – … Cells, Nanomedicine, and …, 2013

FIX ELISA assay Human FIX antigen was detected by ELISA assay as previously described (Wen et al. 2006, 2007, Sheffield et al. 2004, Sayyar et al. 2012) using the reagents and protocol from Affinity Biologicals Inc. (Ancaster, ON, Canada). Statistical analysis

HEXON ISOLATED FROM SIMIAN ADENOVIRUS SEROTYPE 19, HYPERVARIABLE REGION THEREOF AND CHIMERIC ADENOVIRUS USING THE SAME

K Lee, S Yun, D Koh, H Lee, E Jo – US Patent 20,130,058,897, 2013

A sheep anti-coagulation factor IX antibody (Affinity Biologicals, Canada), used as a primary antibody, was 1:3,000 diluted with 5% skim milk/1×TBST buffer, probed at room temperature for one hour, and washed six times each for 5 min with 1×TBST buffer.

 Novel electric power-driven hydrodynamic injection system for gene delivery: safety and efficacy of human factor IX delivery in rats

T Yokoo, K Kamimura, T Suda, T Kanefuji, M Oda… – Gene Therapy, 2013

A mouse anti-hFIX monoclonal antibody (clone HIX-1, F2645l Sigma, St Louis, MO, USA) was used for plate coating, and the horse radish peroxidase-conjugated goat anti-hFIX polyclonal antibody (GAFIX-HRP; Affinity Biologicals, Ancaster, ON, Canada) was used as the…

2012

A potent anticoagulant aptamer directed against factor IXa blocks macromolecular substrate interaction.

B Sullenger, R Woodruff… – Journal of Biological Chemistry, 2012

glycol. Dansyl-Glu-Gly-Arg chloromethylketone (DEGR-CK) was purchased from Calbiochem (EMD Biosciences, San Diego, CA). Anti-factor IX antibodies were purchased from Affinity Biologicals (Ancaster, ON). Unless otherwise

Ribosome readthrough accounts for secreted full‐length factor IX in hemophilia B patients with nonsense mutations

M Pinotti, P Caruso, A Canella, M Campioni… – Human Mutation, 2012

previously described [Belvini et al, 2005] whereas FIX protein levels were evaluated by ELISA (Affinity Biologicals, Ancaster, Canada). (Affinity Biologicals) and the Supersignal® West Femto reagent (Thermo Scientific, Rockford, IL) for detection.

Targeting of the Human Coagulation Factor IX Gene at rDNA Locus of Human Embryonic Stem Cells

X Liu, Y Wu, Z Li, J Yang, J Xue, Y Hu, M Feng… – PLoS ONE, 2012

Blots were incubated with a primary antibody anti-human Factor IX (Affinity Biologicals, Canada) overnight at 4°C. The blots were than incubated with horseradish peroxidase-conjugated secondary antibodies(Sigma, St Louis, MO, US) for 1 hour at room temperature and

METHODS AND COMPOSITIONS FOR TREATING HEMOPHILIA B

PD Gregory, KA High, MC Holmes… – US Patent 20,120,128,635, 2012

Disclosed herein are methods and compositions for insertion of Factor IX (FIX) sequences into the genome of a cell for treating hemophilia B. Quantification of plasma hFIX was performed using an hFIX ELISA kit (Affinity Biologicals)…

2011

Oral Administration Of Therapeutic Agent Coupled To Transporting Agent

G Hortelano… – US Patent 20,110,301,097, 2011
Adult immunocompetent C57BL/6 mice (20 weeks of age) were each fed 100 μg of DNA nanoparticles orally (n=3). Mice were bled regularly, and the concentration of hFIX in plasma determined by ELISA (Affinity Biologicals).

Methods and Compositions for use in Gene Therapy for Treatment of Hemophilia

KA High… – US Patent 20,110,263,690, 2011
for 1 hour. Tissue sections were incubated overnight in the presence of an affinity
purified goat anti-human F.IX antibody (Affinity Biologicals, Hamilton, Ontario, Canada)that was diluted 1:1000 in PBS/1% BSA. After three washes

Engineered factor IX variants bypass FVIII and correct hemophilia A phenotype in mice.

P Milanov, L Ivanciu, D Abriss, P Quade-Lyssy… – Blood, 2011
St Louis, MO) was used as a capture antibody. Peroxidase-conjugated polyclonal goat anti–hFIX (Affinity Biologicals, Hamilton, ON) was used as the detecting antibody 13. For visualization of activated or not activated FIX heavy chain, 300 ng protein of either wild type

HUMAN FACTOR IX VARIANTS WITH AN EXTENDED HALF LIFE

DW Stafford, DM Mann… – US Patent 20,110,154,516, 2011
The amount of FIX remaining in the circulation was measured by ELISA using wild type FIX as a standard. Antibodies for the ELISA were obtained from Affinity Biologicals (Product numbers SAFIX-AP SAFIX-APHRP). The curve was fit to one exponential decay.

 

Transgene loss and changes in the promoter methylation status as determinants for expression duration in non-viral gene transfer for factor IX

J Schuettrumpf, P Milanov, D Abriss… – Human gene …, 2011 – liebertonline.com Sigma-Aldrich, St. Louis, MO) was used as a capture antibody. Peroxidase-conjugated polyclonal goat anti-human FIX (Affinity Biologicals, Hamilton, ON, Canada) was used as the detecting antibody. FIX functional activity was

2010

Peripheral transvenular delivery of adeno-associated viral vectors to skeletal muscle as a novel therapy for hemophilia B

VR Arruda, HH Stedman, V Haurigot… – …, 2010 – bloodjournal.hematologylibrary.org
determined as previously described. 27 Canine FIX (cFIX) antigen concentration was
determined by enzyme-linked immunosorbent assay (ELISA) using matched-pair
antibodies to cFIX (Affinity Biologicals). FIX clotting activity was

Mutant Macaque Factor IX T262A: A Tool for Hemophilia B Gene Therapy Studies in Macaques

A Ishiwata, J Mimuro, H Mizukami… – Thrombosis …, 2010 – Elsevier
Monoclonal antibody 3A6-bound human FIX or macaque FIX was detected with a horseradish peroxidase (HRP)-conjugated goat anti-human FIX polyclonal antibody (Affinity Biologicals Inc., Hamilton, Ontario, Canada). Purified human FIX was used as a standard.

Kinetics and Longevity of Ï•C31 Integrase in Mouse Liver and Cultured Cells

CL Chavez, A Keravala, LE Woodard… – Human gene …, 2010 – liebertonline.com
no. 11-867-423-001; Roche), goat anti-hFIX (GAFIX-AP; Affinity Biologicals, Ancaster, ON, Canada)…

Factor IX ectopically expressed in platelets can be stored in α-granules and corrects the phenotype of hemophilia B mice

G Zhang, Q Shi, SA Fahs… – …, 2010 – bloodjournal.hematologylibrary.org
An affinity-purified horseradish peroxidase-conjugated goat-anti-hFIX polyclonal
antibody (Affinity Biologicals) was used as a detecting antibody.

The Effects of Medium Supplement on High-Level Production of Recombinant Human Factor IX in CHO Cell

IH Lim, JS Kim, G Lee, M Choi… – Cells and Culture, 2010 – Springer
C. Cell concentration dur- ing culture was measured using a hemocytometer by
tryphan blue exclusion method. The secreted rhFIX protein was determined using
sandwich ELISA assay (Affinity Biologicals, Ontario, Canada).

A role for factor XIIa–mediated factor XI activation in thrombus formation in vivo

Q Cheng, EI Tucker, MS Pine… – …, 2010 – bloodjournal.hematologylibrary.org
Samples were size-fractionated on 10% nonreducing SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes. Chemiluminescent Western blotting was performed using goat anti-human fIX IgG (Affinity Biologicals). HK-fXI binding assay.

Interaction of Bacteroides spp. with the kallikrein-kinin system

EC Murphy, M Morgelin, JC Cooney… – Microbiology, 2011 – Soc General Microbiol
goat anti-FXI from Affinity Biologicals Inc.

Use of recombinant adeno-associated virus in the manufacture of a medicament for gene therapy via muscle cells

JM Wilson… – EP Patent 1,696,036, 2010 – freepatentsonline.com
serum albumin (BSA) for 1 hour. Sections were subsequently incubated overnight
with an affinity purified goat anti-human F.IX antibody (Affinity Biologicals) that was
diluted 1:1000 in PBS/1% BSA. After three washes (10 minutes

Oral delivery of bioencapsulated coagulation factor IX prevents inhibitor formation and fatal anaphylaxis in hemophilia B mice

D Verma, B Moghimi, PA LoDuca… – Proceedings of the …, 2010 – National Acad Sciences
Sections were blocked with 2% donkey serum in PBS for 30 min. Goat α-hF.IX (1:400; Affinity Biologicals)

Safety of AAV factor IX peripheral transvenular gene delivery to muscle in hemophilia B dogs

V Haurigot, F Mingozzi, G Buchlis, DJ Hui… – Molecular …, 2010 – nature.com
cFIX antigen detection. cFIX antigen levels were measured by immunoassay using a polyclonal matched-pair antibody set for cFIX enzyme-linked immunosorbent assay (Affinity Biologicals, Ancaster, Ontario, Canada) following manufacturer’s instructions.

LONG-ACTING COAGULATION FACTORS AND METHODS OF PRODUCING SAME

UE Fima… – US Patent App. 12/826,754, 2010 – Google Patents
[0017] FIG. 3 A is a bar graph showing harvests of limited diluted transfected and selected cells with FIX-CTP and FIX- CTP-CTP variants in the presence of 5 (xg/ml ofVitamin K3. The level of FIX was quantified using Human FIX ELISA kit (Affinity Biologicals; cat. No.

2009

Factor IX mutants with enhanced catalytic activity

R Hartmann, M Dockal… – … of Thrombosis and …, 2009 – Wiley Online Library
an anti-FIX enzyme-linked immunosorbent assay, using affinity-purified sheep anti-human FIX IgG (SAFIX-AP; Affinity Biologicals, Ancaster, ON

Molecular characterization of in-frame and out-of-frame alternative splicings in coagulation factor XI pre-mRNA

R Asselta, V Rimoldi, I Guella… – …, 2010 – bloodjournal.hematologylibrary.org
GAFXI-IG) from Affinity Biologicals, previously adsorbed for 40 minutes at room temperature to 14 µL of magnetic bead-protein G (Dynal Biotech), and incubated

ENTIVIRAL VECTORS ENCODING CLOTTING FACTORS FOR GENE THERAPY

JG Mcarthur, DJ Talbot, AD Simmons… – EP Patent …, 2009 – freepatentsonline.com
5X with BBST and incubated with HRP conjugated goat anti-human factor IX antibody (1:100) (Affinity Biologicals, Hamilton, Canada) for