Factor VII ELISA Kit

Factor VII ELISA Kit

The VisuLize Factor VII ELISA Kit is an Enzyme Immunoassay for the quantitative determination of Factor VII antigen in human plasma samples using the double antibody enzyme linked immuno-sorbent assay (ELISA).  This Factor VII ELISA kit is for Research Use Only and must not be used for diagnostic applications.

Product Code: FVII-AG

Features:

  • Rapid sandwich ELISA to measure Factor VII antigen (FVII:Ag)
  • Detection limit of <0.01 IU/mL (<1 %) FVII antigen
  • Rapid sandwich ELISA to measure Factor VII antigen (FVII:Ag)
  • 2 hour total incubation time
  • FVII:Ag reported as IU/ml traceable to ISTH SSC standard for FVII activity
  • Includes normal and low control
  • Intra-assay precision: 4.92 %

Kit Contents:

  • Pre-coated plate in a convenient 96 well; 6×16 strip format
  • Calibrator plasma, Control A and Control B plasmas (2 vials of each)
  • Sample diluent and wash buffer concentrate
  • Ready-to-use detecting antibody, TMB substrate and stop solutions

Species Cross Reactivity: View Chart

Product Datasheet: Factor VII Antigen ELISA Kit


Description of Factor VII – FVII

Factor VII (FVII, also known as Stable Factor and Proconvertin) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FVII is normally ~0.5 μg/ml (10 nM) in plasma. In its zymogen form FVII is a single chain molecule of ~50 kDa. It contains two EGF-like domains and an amino-terminal domain containing 10 γ-carboxyglutamic acid (Gla) residues. These Gla residues allow FVII to bind divalent metal ions and participate in calcium-dependent binding interactions. FVII and activated FVII (FVIIa) bind to tissue factor exposed at the site of vascular injury. FIXa, FXa or FVIIa rapidly activate tissue factor-bound FVII to FVIIa in the presence of calcium and phospholipid. Thrombin and FXIIa are able to activate FVII in the fluid phase in the absence of cofactors. The activation of the single chain zymogen FVII occurs by proteolysis after residue Arg152, resulting in a two-chain active serine protease consisting of a 30 kDa heavy chain and a 18 kDa light chain. In complex with tissue factor, phospholipid and calcium, FVIIa is able to activate F.X and F.IX. Free FVIIa in plasma is remarkably stable, but the activity of FVIIa/TF complex is regulated by Tissue Factor Pathway Inhibitor (TFPI) in the presence of F.Xa, and also by Antithrombin (ATIII) in the presence of heparin1-3.

References and Review

  1. Rao LVM, Bajaj SP, Rapaport SI; Activation of Human Factor VII During Clotting in Vitro; Blood 65, pp 218-226, 1985.
  2. Lawson, JH, Butenas S, Ribarik N, Mann KG; Complex-dependent Inhibition of Factor VIIa by Antithrombin III and Heparin; JBC 268 pp 767- 770, 1993.
  3. Nemerson Y, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 81-93, J.B. Lippincott Co., Philadelphia PA, USA, 1994.

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