2012

Unifying the mechanism of recombinant FVIIa action: dose-dependence is regulated differently by tissue factor and phospholipids.

AM Shibeko, SA Woodle… – 2012

… Materials and methods Human congenital FVII- or FVIII-deficient plasma was from HRF, Inc (Durham, NC). Immunodepleted FVII and FVIII double-deficient plasma was from Affinity Biologicals (Ontario, Canada). Recombinant activated factor VII (rFVIIa) was from Novo Nordisk …

2011

AAV-mediated gene transfer in the perinatal period results in expression of FVII at levels that protect against fatal spontaneous haemorrhage

C Binny, J McIntosh, M Della Peruta, H Kymalainen… – 2011
….secondary antibody was a sheep anti- human FVII polyclonal antibody (Affinity Biologicals, Product No. SAFVII-HRP). Binding was visualized using OPD substrate, reading absorbance at 490 nm. Standards …

Relationships of plasma factor VIIa-antithrombin complexes to manifest and future cardiovascular disease

A Silveira, D Scanavini, S Boquist, CG Ericsson… – Thrombosis Research, 2011
… The First International Standard VIIa Concentrate (National Institute for Biological Standards and Control, Hertfordshire, UK) was used as calibrator. Total factor VII in plasma was determined as VIIag, in a sandwich ELISA with antibodies from Affinity Biologicals Inc. …

2009

M Pinotti, D Balestra, L Rizzotto… – …, 2009 – bloodjournal.hematologylibrary.org
… FVII protein levels in medium were measured by a commercially available enzyme-linked immunosorbent assay (ELISA) kit (FVII-EIA; Affinity Biologicals, Ancaster, ON). …