Factor X (F10, FX) ELISA Kit
The VisuLize Factor X (F10, FX) ELISA Kit is an Enzyme Immunoassay for the quantitative determination of Factor X antigen in human plasma samples using the double antibody enzyme linked immuno-sorbent assay (ELISA). The kit includes: pre-coated (Capture Antibody) ELISA plate, Detecting Antibody, Calibrator Plasma, Control A and Control B Plasma, Wash Buffer, Sample Diluent, TMB Substrate and Stop Solution.
Factor X (F10, FX) ELISA kit principles:
Strip wells are pre-coated with sheep polyclonal antibody to human Factor VII. Plasma samples are diluted and applied to the wells. The FX antigen present binds to the coated antibody. After washing away unbound material, peroxidase labeled sheep detecting antibody is applied and allowed to bind to the captured FX. The wells are again washed and a solution of TMB (the peroxidase substrate tetramethylbenzidine) is applied and allowed to react for a fixed period of time. A blue color develops which changes to yellow upon quenching the reaction with acid. The color formed is measured spectrophotometrically in a microplate reader at 450nm. The absorbance at 450nm is directly proportional to the quantity of FX antigen captured onto the well. The assay is calibrated using the calibrator plasma provided in the kit.