Factor XIII Deficient Plasma
Affinity’s Factor XIII Deficient Plasma is manufactured from pooled normal human plasma depleted of Factor XIII using antibodies directed to Factor XII. Only the highest quality citrated plasma is used as starting material. Our Frozen Deficient or Depleted Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and our Lyophilized Deficient Plasma products are typically buffered with the addition of HEPES to 50mM final concentration.
All Factor XIII Deficient or Depleted Plasma from Affinity Biologicals are for Research Use Only and must not be used for diagnostic applications.
Factor XIII Deficient – Depleted Plasma
Factor XIII Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Factor XIII by selective affinity immuno-adsorption using antibodies directed towards FXIII. Available in two different formats – Frozen and Lyophilized
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and are available in 1ml vials to litre quantities. Our Factor XIII Deficient – Depleted Plasma can be used for further manufacturing or research use only applications.
Frozen Factor XIII Deficient Plasma
Product Code: FXIII-DP
Presentation: Frozen Factor XIII Deficient – Depleted Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Certificate of Analysis: available upon request
Lyophilized Factor XIII Deficient Plasma
Product Code: FXIII-LDP
Presentation: Freeze Dried Factor XIII Deficient – Depleted Plasma
Preparation: Pooled normal citrated human plasma depleted of FXIII using antibodies directed to FXIII immobilized on agarose beads. Plasma contains 50 mM HEPES and stabilizers.
Reconstitution: Reconstitute with 1 mL of reagent grade water. Allow contents to dissolve for 15 minutes at room temperature with occasional swirling. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Storage and Stability: Prior to reconstitution, dried plasma should be stored at 2-8°C. Product is stable until date stated on vial label when stored at 2-8oC. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Certificate of Analysis: available upon request
Description of Factor XIII
Factor XIII (FXIII, fibrin stabilizing factor) is the proenzyme form of a transamidase that is essential for normal haemostasis and fibrinolysis, wound healing, female fertility and foetal development. Extracellular F.XIII consists of A subunits (83 kDa each) which contain the enzyme moiety, and B subunits (76 kDa each) which act as a carrier protein for the A subunit in circulation. Both subunits are produced under separate genetic control. In plasma, FXIII exists as a non-covalent tetrameric complex (320 kDa) of two A-subunits and two B-subunits (A2B2). The concentration of F.XIII tetramer in plasma is ~25 μg/ml (~80 nM). An intracellular form of FXIII is found in platelets, megakaryocytes and monocytes. This form of F.XIII presents as a dimer of two A-subunits only and has a molecular weight of 160 kDa. The importance of these intracellular stores is demonstrated by the observation that platelets can contribute up to half of the FXIII activity in platelet rich plasma. The activation of FXIII involves several steps. Thrombin cleaves after Arg37 of each A-subunit in the A2B2 tetramer, releasing a 4.5 kDa activation peptide. Additional conformational changes induced by the binding of calcium, and by dissociation of the B-subunits from the A-subunit dimer are required to obtain full enzyme activity. FXIIIa is a cysteine protease that catalyses the formation of γ-glutamyl-ε-lysyl bonds between the γ and α chains of polymerised fibrin molecules. Other proteins found crosslinked into fibrin clots by FXIIIa include fibrinogen, α2antiplasmin, fibronectin, vitronectin and von Willebrand factor 1-3.
References and Reviews
- McDonagh J; Structure and Function of Factor XIII; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 301-313, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Inbal A, Muszbek L; Coagulation Factor Deficiencies and Pregnancy Loss; Seminars in Thrombosis and Haemostasis 29, pp 171-174, 2003.
- Murdock PJ, Owens DL, Chitolie A, Hutton RA, Lee CA; Development and Evaluation of ELISAs for Factor XIIIA and XIIIB Subunits in Plasma; Thrombosis Research 67, pp 73-79, 1992.
SAFXIII-IG – Sheep anti human Factor XIII, purified IgG (10mg vial)
FXIII-EIA – Factor XIII Paired Antibody Set – 5 plate set
SAFXIII-HRP – Sheep anti human Factor XIII, peroxidase conjugated IgG (0.2 mg vial)
SAF13A-HRP – Sheep anti human Factor XIII (A subunit), peroxidase conjugated IgG (0.2 mg vial)
FXIII-DP – Frozen Factor XIII Deficient Plasma – 1ml or bulk quantities
FXIII-LDP – Lyophilized Factor XIII Deficient Plasma – 1ml
SAF13A-IG – Sheep anti human Factor XIII (A-subunit), purified IgG (10mg vial)
SAF13A-AP – Sheep anti human Factor XIII (A-subunit), affinity purified IgG (0.5 mg vial)