Fibrinogen – Published Papers Using Affinity Biologicals Products

2013

[HTML] A Novel Role for Pro-Coagulant Microvesicles in the Early Host Defense against Streptococcus pyogenes

S Oehmcke, J Westman, J Malmström, M Mörgelin… – PLoS Pathogens, 2013
150 µl bacteria and 30 µl MVs – in the presence or absence of 300 µl human plasma – were mixed and incubated for 30 min. at 37°C. Alternatively, fibrinogen depleted plasma (Affinity Biologicals, Canada) was used. Bacteria … 
 

Effects of Caffeine and Stress on Biomarkers of Cardiovascular Disease in Healthy Men and Women with a Family History of Hypertension

JM Bennett, IM Rodrigues, LC Klein – Stress and Health, 2013
of 4000 ng/mL. Fibrinogen levels were assessed using an ELISA (Affinity Biologicals, Inc., Ancaster, Ontario, Canada); this kit has a minimum sensitivity of 0.003 mg/mL and an upper limit of 200 mg/mL. Cortisol, estradiol and

2011

Thrombocytopenia after pig‐to‐baboon liver xenotransplantation: where do platelets go?

M Ezzelarab, B Ekser, B Gridelli, H Iwase, D Ayares… – Xenotransplantation, 2011
Cryosections of stored liver biopsies were cut (8 μm), as previously described. Platelets were stained using mouse anti-human CD41 (Abcam, Cambridge, MA, USA), and fibrin was stained using sheep anti-human fibrin antibody (Affinity Biologicals, Ancaster, ON, Canada).

The procoagulant properties of purified fibrinogen concentrate are enhanced by carbon monoxide releasing molecule-2 

KA Machovec, DS Ushakumari, IJ Welsby… – Thrombosis Research, 2011
Materials and methods. Fibrinogen deficient plasma experiments. Fibrinogen deficient
plasma anticoagulated with sodium citrate was obtained from a commercially available
source (Affinity Biologicals, Inc., Ancaster, Ontario, Canada).

NEW ROLE OF FXIIa IN FIBRIN STRUCTURE - Factor XIIa regulates the structure of the fibrin clot independently of thrombin generation through direct interaction with fibrin

Authors: Joke Konings 1, Jose… Blood First Edition Paper, prepublished online August 9, 2011; DOI 10.1182/blood-2011-03-339572…Factor XIIa regulates the structure of the fibrin clot independently of thrombin generation through direct interaction with fibrin RunningPolyclonal sheep anti human fibrinogen and polyclonal goat anti human FXII were from Affinity Biologicals (Ancaster, Canada)

Age-Related Differences in Plasma Proteins: How Plasma Proteins Change from Neonates to Adults

V Ignjatovic, C Lai, R Summerhayes, U Mathesius… – PloS one, 2011 – dx.plos.org the sheep anti-human fibrinogen peroxidase conjugated IgG polyclonal antibody (SAFG-APHRP) or the goat anti-human alpha-2-macroglobulin peroxidase conjugated IgG monoclonal antibody (GAA2M-APHRP), at a dilution of 1:10000 (Affinity Biologicals, Ontario, Canada).

DL Sauls, M Warren… – Thrombosis Research, 2011 – Elsevier
Fibrinogen was visualized using a polyclonal sheep anti-human fibrinogen conjugated to HRP (Affinity Biologicals), a monoclonal antibody to the D-Domain of fibrinogen (Accurate Chemical Co, Westbury, NJ, NYB Ea3, directed against an epitope in the Bβ chain 134-461), or a

2010

VG Nielsen, SN Malayaman, ES Khan… – Blood Coagulation & …, 2010 – journals.lww.com
experimentation. Fibrinogen-deficient plasma (Affinity Biologicals, Inc., Ancaster, Ontario, Canada) anticoagulated with sodium citrate was used in experiments investigating the effects of CORM-2 on fibrinogen. This plasma

SJAC Peters, T Vanhaecke, P Papeleu… – Cell and tissue …, 2010 – Springer
… sheep anti-rat fibrinogen conjugated to horseradish peroxidase (HRP) were purchased from Affinity Biologicals (Ancaster, Ontario, Canada), fibrinogen from rat plasma was from Sigma and sheep anti-rat albumin, sheep anti-rat albumin HRP-labelled and rat albumin antigens were from Biogenesis

ACA Cleuren, IK van der Linden… – … of Thrombosis and …, 2010 – Wiley Online Library according to the manufacturer’s protocol. Plasma antithrombin antigen and fibrinogen antigen levels were assessed by using commercial murine enzyme-linked immunosorbent assay kits from Affinity Biologicals (Ancaster, Canada).

Oversulfated Heparin By-Products Induce Thrombin Generation in Human Plasmas Through Contact System Activation

Y Qian, J Pan, X Zhou, DE Hourcade… – Clinical and Applied …, 2010 – cat.sagepub.com
Thrombin then cleaves fibrinogen to form fibrin LC/ MS) analysis.10 Oversulfated HB was chemically sulfated and analyzed using both CE and NMR in our laboratory as described. All factor-depleted plasmas and antibodies used in this study were from Affinity Biologicals, Inc

RD Murray, G Wieringa, JA Lawrance… – Clinical …, 2010 – Wiley Online Library
Intra-assay CVs were 5·1% at 5·2 ng/ml and 6·8% at 48·3 ng/ml respectively. Inter-assay CVs were 14·3% at 4·3 ng/ml and 11·6% at 31·0 ng/ml respectively. Fibrinogen was estimated using ELISA (Affinity Biologicals, ON, Canada). Inter- and intra-assay CVs were …

2009

RD Murray, G Wieringa, JA Lawrance… – Clinical …, 2010 – Wiley Online Library Fibrinogen was estimated using ELISA (Affinity Biologicals, ON, Canada).