Antibodies to Heparin CoFactor II (HCII)

Heparin CoFactor II, human

Antibody Host Catalogue No. Size Product Insert
purified IgG Goat GAHC2-IG 5 mg
affinity purified IgG Goat GAHC2-AP 0.5 mg
affinity purified peroxidase conj. IgG Goat GAHC2-APHRP 1 mg
Matched Pair Antibodies
– For EIA of Heparin CoFactor II HCII-EIA 5 plates
– For EIA of Thrombin-HCII Complex THCII-EIA 5 plates

Description of Heparin CoFactor II (HCII)

Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 μg/ml (0.8-1.5 μM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.

References:

1. Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.

2. Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.

3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.

Related Products:

GAHC2-IG Goat anti-Heparin Cofactor II, IgG from antiserum

GAHC2-APHRP Goat anti- Heparin Cofactor II, APIgG-peroxidase

SAHC2-IG Sheep anti-HCII, whole IgG from antiserum

HCII-EIA Paired antibody set for ELISA of HCII, 4 x 96 wells

THCII-EIA Paired antibody ELISA of Thrombin-HCII complexes

HCII-DP Human plasma deficient in Heparin CoFactor II, immune depleted

ATHC-DP Human ATIII & HCII deficient plasma, immune depleted, Frozen

ATHC-LDP Human ATIII & HCII def plasma, immune depleted, Lyophilized