Heparin CoFactor II, human
Description of Heparin CoFactor II (HCII)
Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 ÃŽÂ¼g/ml (0.8-1.5 ÃŽÂ¼M). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.
1. Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.
2. Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.
3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.
GAHC2-IG Goat anti-Heparin Cofactor II, IgG from antiserum
GAHC2-APHRP Goat anti- Heparin Cofactor II, APIgG-peroxidase
SAHC2-IG Sheep anti-HCII, whole IgG from antiserum
HCII-EIA Paired antibody set for ELISA of HCII, 4 x 96 wells
THCII-EIA Paired antibody ELISA of Thrombin-HCII complexes
HCII-DP Human plasma deficient in Heparin CoFactor II, immune depleted
ATHC-DP Human ATIII & HCII deficient plasma, immune depleted, Frozen
ATHC-LDP Human ATIII & HCII def plasma, immune depleted, Lyophilized