Heparin CoFactor II – HCII Antibodies
Affinity Biologicals, Inc. manufactures a broad range of Heparin CoFactor II – HCII Antibodies which can be found in the listing below. Further information about each individual Heparin CoFactor II – HCII Antibody is available by following the associated links. Our Heparin CoFactor II – HCII Antibodies are excellent for use in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays. These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins. Affinity’s Heparin CoFactor II – HCII Antibodies are manufactured for use in research applications.
Listing of Heparin CoFactor II – HCII Antibodies
GAHC2-IG – Goat anti human Heparin Cofactor II, purified IgG (5mg vial)
GAHC2-APHRP – Goat anti human Heparin Cofactor II, affinity purified, peroxidase conj. IgG (0.1 mg vial)
GAHC2-AP – Goat anti human Heparin Cofactor II, affinity purified IgG (0.5 mg vial)
Description of Heparin CoFactor II (HCII)
Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 μg/ml (0.8-1.5 μM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.
References and Reviews
- Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.
- Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.
- Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.