Affinity Biologicals, Inc.
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      • Factor VII ELISA Kit
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      • Factor XI ELISA Kit
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    • Custom Polyclonal Antibody Production
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    • FAQ – Frequently Asked Questions
    • ELISA and Immunoblotting Methods
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    • Polyclonal Antibody Formats
    • Antibody Applications
    • Antibody Concentration Ranges
    • Antibody Storage – Handling Recommendations
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Matched-Pair Antibody Sets for ELISA – EIA

The following products consist of matched capture and detecting antibodies for the preparation of ELISA assays for most coagulation and hemostasis related antigens. The product insert provides recipes and sources for materials required but not provided. The VisuCal Antigen Calibrator Plasma and the VisuLize Buffer Pak are ancillary products to our Matched-Pair Antibody Sets to aid in the preparation and performance of ELISA assays. These products are intended to facilitate the end user in establishing an in-house immunoassay for Research Use Only and must not be used for diagnostic applications. Assay validation is the responsibility of the end user.

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Individual Matched Pair Antibody Set – Listing

DescriptionCatalogue No.Size
FibrinogenFG-EIA5 plates
Murine FibrinogenMFG-EIA4 plates
Prothrombin - Factor IIFII-EIA5 plates
Factor VFV-EIA5 plates
Factor VIIFVII-EIA5 plates
Factor VIII:CF8C-EIA4 plates
Canine Factor VIII:CCFVIII-EIA4 plates
vWF - von Willebrand FactorVWF-EIA5 plates
Factor IXFIX-EIA5 plates
Canine Factor IXCFIX-EIA4 plates
Factor XFX-EIA5 plates
Murine Factor XMFX-EIA4 plates
Factor XIFXI-EIA5 plates
Factor XIIFXII-EIA5 plates
PrekallikreinPK-EIA4 plates
Factor XIIIFXIII-EIA5 plates
Protein CPC-EIA5 plates
Alpha-1-AntitrypsinA1AT-EIA5 plates
Protein SPS-EIA5 plates
Protein C InhibitorPCI-EIA5 plates
ATIII - AntithrombinATIII-EIA5 plates
TAT - Thrombin - Antithrombin ComplexTAT-EIA5 plates
Beta-2-Glycoprotein-1 (APOH)APOH-EIA5 plates
PlasminogenPG-EIA5 plates
tPA - Tissue Plasminogen ActivatorTPA-EIA5 plates
Neutralization of ATIIIAT-NU1010 det.
EIA Accessories
VisuLize Buffer PakEIA-PAK-15 plates
VisuCal Antigen Calibrator PlasmaEIA-CSA1ml or 5 ml

Description Catalogue No. Size
Fibrinogen FG-EIA 5 plates
Rabbit Fibrinogen RFG-EIA 4 plates
Murine Fibrinogen MFG-EIA 4 plates
Prothrombin - Factor II FII-EIA 5 plates
Factor V FV-EIA 5 plates
Factor VII FVII-EIA 5 plates
Factor VIII:C F8C-EIA 4 plates
Canine Factor VIII:C CFVIII-EIA 4 plates
vWF - von Willebrand Factor VWF-EIA 5 plates
Factor IX FIX-EIA 5 plates
Canine Factor IX CFIX-EIA 4 plates
Factor X FX-EIA 5 plates
Murine Factor X MFX-EIA 4 plates
Factor XI FXI-EIA 5 plates
Factor XII FXII-EIA 5 plates
Prekallikrein PK-EIA 4 plates
Factor XIII FXIII-EIA 5 plates
Protein C PC-EIA 5 plates
APC - Protein C Inhibitor Complex APCPCI-EIA 5 plates
Alpha-1-Antitrypsin A1AT-EIA 5 plates
Protein S PS-EIA 5 plates
Protein C Inhibitor PCI-EIA 5 plates
ATIII - Antithrombin ATIII-EIA 5 plates
Murine Antithrombin MATIII-EIA 4 plates
TAT - Thrombin - Antithrombin Complex TAT-EIA 5 plates
HCII - Heparin Cofactor II HCIII-EIA 4 plates
Thrombin - HCII Complex THCII-EIA 5 plates
Beta-2-Glycoprotein-1 (APOH) APOH-EIA 5 plates
Plasminogen PG-EIA 5 plates
Murine Plasminogen MPG-EIA 4 plates
tPA - Tissue Plasminogen Activator TPA-EIA 5 plates
Neutralization of ATIII AT-NU10 10 det.
EIA Accessories
VisuLize Buffer Pak EIA-PAK-1 5 plates
VisuCal Antigen Calibrator Plasma EIA-CSA 1ml or 5 ml
Matched-Pair Antibody Sets for ELISA - EIA Sets

VisuLize Buffer Pak

Our Buffer Pak consists of assay plates, general purpose buffers and materials for the preparation and performance of ELISA assays. This Buffer Pak provides sufficient reagents for 5 x 96 well assays.

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Matched-Pair Antibody Sets for ELISA - EIA Sets

VisuCal Antigen Calibrator Plasma

This Antigen Calibrator Plasma is a lyophilized, citrated reference plasma intended for use in the calibration of Research Use Only antigen assays for Factor II, Factor VII, Factor X, Factor XI, Factor XIII, Protein C, Protein S, ATIII, vWF and Fibrinogen.

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Further information about our antibody products can be found below:

  • Polyclonal Antibody Formats

    For reasons of price and performance, polyclonal antibodies are often the reagents of choice in laboratory applications. Affinity Biologicals offers an extensive line of antibodies to haemostasis related proteins. Although ABI does offer some murine monoclonal antibodies, most antibodies are polyclonal antibodies raised in sheep and goats. Because polyclonal antibodies contain antibodies to multiple recognition sites (epitopes) on an antigen the apparent affinity and avidity of antigen-antibody interactions is very high. This results in greater sensitivity and also means that the antigen is more likely to be recognized after denaturation or proteolytic processing. These are important considerations for applications such as immunoblotting from denaturing gels such as SDS-PAGE and in immunohistochemistry of fixed cells and tissues. Polyclonal antibodies are excellent for use in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunoprecipitation techniques and activity neutralization assays. At ABI we have had good success using our polyclonal antibodies immobilized to resins to specifically immuno-deplete target proteins from citrated plasma and to recover the target protein from these resins with excellent recovery of activity.

    Most Affinity Biologicals antibodies are available in several different formats, each differing in the level of refinement.

    • Whole IgG is the base level product and refers to IgG antibodies purified from antiserum (usually sheep or goat) using multiple precipitation and dialysis steps. The purity of the IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one millilitre of antiserum. This level of product is generally intended for use in applications such as immunoprecipitation, immunoelectrophoresis, immuno-depletion and activity neutralization assays.
    • Affinity-purified IgG are antibodies further purified using immobilized antigen. Affinity-purification is an enrichment process in which whole IgG is passed over a resin to which antigen has been covalently attached. Non-reactive antibodies are washed away and specific antibodies are eluted under conditions that disrupt the antigen-antibody interaction. The result is a very high-purity product with a substantially higher titre than whole IgG. This level of product is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.
    • Enzyme labeled IgG is either whole IgG or affinity-purified IgG that has been conjugated with Horseradish Peroxidase as a reporter enzyme. These products are intended for use as labeled primary antibodies in applications such as immunoassay and immunoblotting.
  • Polyclonal Antibody Applications

    Polyclonal antibodies typically recognize numerous binding sites on protein antigens, which in concert produce very high affinity binding constants. In many cases, antigens are recognized in denaturing as well as non-denaturing conditions. In general, applications in which polyclonal antibodies work well include:

    •   ELISA (direct, double antibody or competitive)
    •   Gel Precipitation (immunoelectrophoresis, immunodiffusion)
    •   Neutralization of antigen activity
    •   Depletion of antigen by immune-affinity chromatography
    •   Immunoblotting (Western blotting) from SDS-PAGE
    •   Immunostaining (ImmunoHistoChemistry)
    •   Flow cytometry

    It should be noted that while many of our antibodies may be suitable in the above applications, only direct ELISA and Gel Precipitation testing is done on every lot of antibody as part of product quality control and approval.  ABI will guarantee performance in direct ELISA and Gel Precipitation applications only, except where otherwise indicated.

  • Antibody Concentration Ranges for ELISA, Immunoblotting and Immunohistochemistry Applications

    The optimal concentration of antibody for any particular application will vary depending on the antibody format as well as the detection method used. ABI strongly suggests that each antibody be titrated by the end user to determine the optimal working concentration for each particular application. However, based on our experience we can make some general recommendations regarding antibody concentration ranges that can be used as a starting point in optimizing assay conditions.

    The following chart contains general recommendations only and may not apply to all our products.

    Format Immunoblotting (ECL detection) ELISA (capture) ELISA (detecting)
    Whole IgG 5-25 ug/ml 20-50 ug/ml NA
    Affinity-Purified (AP-IgG) 0.5-5 ug/ml 10 ug/ml NA
    IgG Peroxidase (HRP) 5 ug/ml NA 10 ug/ml
    AP-IgG Peroxidase (AP-HRP) 1 ug/ml NA 2 ug/ml
  • Polyclonal Antibody Storage - Handling Recommendations

    All antibody products are dispensed with a 5% excess volume.

    ABI antibodies are supplied in a solution of HBS-glycerol (10mM HEPES, 0.15M NaCl, pH 7.4, 50% glycerol (v/v)) to increase the long-term stability of the IgG. The glycerol acts to stabilize the IgG by reducing hydration and also helps to suppress microbial growth. We recommend storage between -10°C and -20°C. At these temperatures the solution will not freeze. The solution of HBS-glycerol allows multiple withdrawals from the vial without the need for numerous freeze-thaw cycles that reduce antibody potency. We recommend that the vial be warmed to ambient temperature and gently mixed before pipetting to reduce the viscosity. The antibodies are supplied in microcentrifuge tubes and residual reagent may be gathered from the walls and cap with a brief centrifugation. Glycerol is hygroscopic and therefore care should be taken to ensure that caps are re-fastened securely with the O-ring intact before putting the vial back in the freezer.

    Any of our antibodies can usually be dialyzed into any buffer according to customer specifications. A special handling fee of 20% of the purchase price applies to orders of 10 vials or less.

Find out who is using our products?

View a listing of important journal articles where Affinity Biologicals products have been used.
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  • TAT Complex Paired Antibody Set

    TAT Complex Paired Antibody Set

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    TAT-EIA – Thrombin Antithrombin – TAT Complex Paired Antibody Set – 5 plate set

  • tPA Paired Antibodies

    tPA Paired Antibody Set

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    TPA-EIA – Tissue Plasminogen Activator – tPA Paired Antibody Set – 5 plate set

  • Buffer Pak for paired antibodies

    Visulize Buffer Pak

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    EIA-PAK-1 – VisuLize Buffer Pak – 5 plates

  • Website Antigen Calibrator pic

    Antigen Calibrator Plasma

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    EIA-CSA-1 – VisuCal Antigen Calibrator Plasma 1ml vial
    EIA-CSA-5 – VisuCal Antigen Calibrator Plasma 5x1ml vial package

  • Fibrinogen Paired Antibody Set

    Fibrinogen Paired Antibody Set

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    FG-EIA – Fibrinogen Paired Antibody Set – 5 plate set

  • Prothrombin - Factor II Paired Antibody Set

    Prothrombin – Factor II Paired Antibody Set

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    FII-EIA – Prothrombin – Factor II Paired Antibody Set – 5 plate set

Affinity Biologicals, Inc.
+1-905-304-9896
+1-800-903-6020Can/US
info@affinitybiologicals.com
1348 Sandhill Drive
Ancaster, ON
L9G 4V5

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