APC-PCI Complex Paired Antibody Set
Affinity’s APC-PCI Complex Paired Antibody Set consists of matched capture and detecting antibodies that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for five full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This APC-PCI Complex Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications. Assay validation is the responsibility of the end user.
Product Code: APCPCI-EIA
- Capture Antibody (APCPCI-EIA-C): One yellow-capped vial containing 0.5 ml of polyclonal affinity purified anti-Protein C antibody for coating plates.
- Detecting Antibody (APCPCI-EIA-D): One red-capped tube containing 0.5 ml of peroxidase conjugated affinity-purified polyclonal anti-Protein C Inhibitor antibody for detection of captured APC-Protein C Inhibitor Complex.
Species Cross Reactivity: View Chart
Description of APC- PCI Complex (APCPCI)
Activation of coagulation leads to the generation of thrombin which, in the presence of thrombomodulin, will activate Protein C to the enzyme activated Protein C (APC). Unless regulated, APC will exert its anticoagulant function through proteolytic inactivation of factor Va and factor VIIIa. In blood, the activity of APC is regulated in part through interaction with protease inhibitors to form inactive enzyme-inhibitor complexes. Based on physiological concentrations and the kinetics of inhibition, the primary inhibitor of APC in blood is Protein
C Inhibitor (PCI, also known as plasminogen activator inhibitor-3), followed by alpha-1-antitrypsin (A1AT, also known as Alpha-1-Proteinase Inhibitor) and Alpha-2-Macroglobulin. The APC-PCI complex (APC-PCI) results when APC cleaves a scissile bond near the C-terminus of PCI, forming a covalent, 1:1 acyl enzyme intermediate with PCI with an apparent mass of 110 kDa. Calcium is not required for this interaction, but the rate of APC inhibition by PCI can be accelerated 50-fold by optimal concentrations of heparin. APC-PCI complex is cleared from circulation with a half-life of 19 minutes, presumably by serpin-enzyme complex receptors on the surface of hepatocytes.1-4
References and Reviews:
- Bauer KA; Laboratory Markers of Coagulation Activation; Arch Pathol Lab Med 117, pp 71-77, 1993.
- Broze GJ, Miletich JP; Biochemistry and Physiology of Protein C, Protein S and Thrombomodulin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 259-276, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Heeb MJ, Mosher D, Griffin JH; Activation and Complexation of Protein C and Cleavage and Decrease of Protein S in Plasma of Patients With Intravascular Coagulation; Blood 73, pp 455-461, 1989.
- Laurell M, Stenflo J, Carlson TH; Turnover of *I-Protein C Inhibitor and *I-α1Antitrypsin and Their Complexes with Activated Protein C; Blood 76, pp 2290-2295, 1990