Donkey anti-sheep IgG – Biotinylated
Affinity’s Donkey anti-sheep IgG – Biotinylated secondary antibody is a donkey immunoglobulin that has been affinity purified on immobilized sheep IgG and conjugated to biotin. The APIgG-biotin concentration is 1 mg/ml in phosphate-buffered saline (PBS) containing 1 mg/ml bovine albumin and 0.1% sodium azide (w/v), pH 7.4. The incorporation of biotin ranges between 5.0 and 9.0 moles biotin per mole IgG as determined by HABA assay. Total protein is 0.5 mg.
Product Code: DAS-APBIO
Retail Product Size: 0.5mg vial
Host Animal: Donkey
Immunogen: Whole IgG (heavy and light chain) purified from sheep serum.
Specificity: Immunoelectrophoresis of this antibody prior to conjugation gave a single precipitin line of identical mobility to that obtained with purified sheep IgG. There is 100% cross-reactivity with goat IgG when tested by ELISA.
Application: This product is suitable as a source of biotinylated antibodies to sheep IgG.