Plasminogen Polyclonal Antibody – Affinity Purified – Goat
Affinity’s Plasminogen Polyclonal Antibody – Affinity Purified – Goat is the highest level of our Plasminogen antibody family. During the Antigen Affinity Purification process the IgG has had any non-specific immunoglobulin fraction eliminated which enriches the specificity of the remaining immunoglobulin towards the target antigen. The result is a very high-purity product with a substantially higher titre than whole or purified IgG. Our Plasminogen Polyclonal Antibody – Affinity Purified – Goat is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v) and is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.
Product Code: GAPG-AP
Retail Product Size: 0.5mg vial
Host Animal: Goat Anti-Human Plasminogen Polyclonal Antibody – Affinity Purified – Goat
Species Cross Reactivity: View Chart
Product Datasheet: Plasminogen Polyclonal Antibody, affinity purified anti-human goat IgG
Description of Plasminogen
Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 μg/ml (~2.3 μM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to α2antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is α2antiplasmin. Other physiological inhibitors of plasmin include α2macroglobulin and antithrombin1-3.
References and Reviews
- Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Castellino FJ, Powell JR; Human Plasminogen; Methods in Enzymology 80, pp 365-378, 1981.
- Wiman B, Collen D; Molecular Mechanism of Physiological Fibrinolysis; Nature 272, pp 548-553, 1978.