Protein C Inhibitor Paired Antibody Set
Affinity’s Protein C Inhibitor Paired Antibody Set consists of matched capture and detecting antibodies that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for five full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This Protein C Inhibitor Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications. Assay validation is the responsibility of the end user.
Product Code: PCI-EIA
- Capture Antibody (PCI-EIA-C): One yellow-capped vial containing 0.5 ml of polyclonal affinity purified anti-Protein C Inhibitor antibody for coating plates.
- Detecting Antibody (PCI-EIA-D): One red-capped tube containing 0.5 ml of peroxidase conjugated affinity-purified polyclonal anti-Protein C Inhibitor antibody for detection of captured Protein C Inhibitor.
Species Cross Reactivity: View Chart
Product Datasheet: Protein C Inhibitor - PAI-3 Matched Pair Antibody Set for ELISA
Description of Protein C Inhibitor (PCI)
Protein C Inhibitor (PCI), also known as Plasminogen Activator Inhibitor 3 (PAI3), is a member of the SERPIN family of proteinase inhibitors. It is produced in the liver as a single chain glycoprotein (mass of 57 kDa) and circulates in plasma at a concentration of 5 μg/ml (~90 nM). PCI is also found in urine in lower concentrations of 250 ng/mL (~0.4 nM). PCI is the primary inhibitor of activated Protein C (APC) in plasma but demonstrates a relatively broad specificity, also inhibiting thrombin, FXa, FXIa, kallikrein, tPA, urokinase, prostate specific antigen, acrosin, chymotrypsin and trypsin. The preferred enzyme target for PCI appears to be thrombin and this interaction is increased by more than 100 fold in the presence of thrombomodulin. Like ATIII and HCII, the inhibitory activity of PCI towards some of these enzymes is stimulated by high concentrations of heparin (5 U/ml) which can accelerate the rate of inactivation as much as 50 fold. Enzyme inhibition by PCI occurs through proteolytic cleavage at Arg354-Ser355 and subsequent rapid formation of a stable, inactive 1:1 enzyme-PCI complex. Interaction with APC results in an SDS-stable APC-PCI complex of 102 kDa.1-4.
References and Reviews
- Suzuki K; Protein C Inhibitor; Methods in Enzymology 222, pp 385-399, 1993.
- Cooper ST, Whinna HC, Jackson TP, Boyd JM, Church FC; Intermolecular Interactions between Protein C Inhibitor and Coagulation Proteases; Biochemistry: 34, pp. 12991-12997, 1995.
- Heeb MJ, Espana F, Geiger M, Collen D, Stump DC, Griffin JH; Immunological Identity of Heparin-dependent Plasma & Urinary Protein C Inhibitor & Plasminogen Activator Inhibitor-3; JBC:262, pp 15813-15816, 1987.
- Rezaie AR, Copper ST, Church FC, Esmon CT; Protein C Inhibitor is a Potent Inhibitor of the Thrombin-Thrombomodulin Complex; JBC:270, pp 25336-25339, 1995.