von Willebrand Factor – vWF Paired Antibody Set
Affinity’s von Willebrand Factor – vWF Paired Antibody Set consists of matched capture and detecting antibodies that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for five full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This von Willebrand Factor – vWF Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications. Assay validation is the responsibility of the end user.
Product Code: VWF-EIA
- Capture Antibody (VWF-EIA-C): One yellow-capped vial containing 0.5 ml of polyclonal affinity purified anti-von Willebrand Factor antibody for coating plates.
- Detecting Antibody (VWF-EIA-D): One red-capped tube containing 0.5 ml of peroxidase conjugated affinity-purified polyclonal anti-von Willebrand Factor antibody for detection of captured vWF.
Species Cross Reactivity: View Chart
Product Datasheet: vWF - von Willebrand Factor Matched Pair Antibody Set for ELISA - VWF-EIA
Description of von Willebrand Factor (vWF)
von Willebrand Factor (vWF, also previously referred to as Factor VIII related antigen) is a large adhesive protein produced in endothelial cells and megakaryocytes. There are two critical functions of vWF, the first being its involvement in the process of platelet adhesion and aggregation through interaction with platelet receptor glycoprotein Ib, the second being the binding and stabilization of Factor VIII (antihemophilic factor) for secretion and transport in plasma. The vWF precursor protein is synthesized with a 95,000 dalton propeptide (also known as vWF antigen-II), believed to be involved in the intracellular multimerization of the vWF subunits. The mature vWF multimers are then packed into storage organelles within the cell (Weibel-Palade bodies) after which the propeptide is cleaved and released. vWF circulates as multimers of disulphide linked 220,000 dalton subunits and the molecular weight of these multimers ranges from 0.5-20 million daltons.
The plasma concentration of vWF is typically 10 μg/ml, but increased levels are often observed in pregnancy and other conditions of physiological stress. von Willebrand’s disease (vWD) is perhaps the most common inherited bleeding disorder in humans and is the result of either quantitative deficiencies of vWF (vWD Types I & III), or one of a number of qualitative disorders of vWF structure and function (vWD Type II).
References and Reviews
- Montgomery RR, Coller BS, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 134-168, J.B. Lippincott Co., Philadelphia PA,.USA, 1994.
- Sadler JE; von Willebrand Factor (Minireview); JBC 266:34, pp 22777-22780, 1991.