Protein C Deficient Plasma
Affinity’s Protein C Deficient Plasma is manufactured from pooled normal human plasma depleted of Protein C using antibodies directed to PC. Only the highest quality citrated plasma is used as starting material. Our Frozen Deficient or Depleted Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and our Lyophilized Deficient Plasma products are typically buffered with the addition of HEPES to 50mM final concentration.
All Protein C Deficient or Depleted Plasma from Affinity Biologicals are for Research Use Only and must not be used for diagnostic applications.
Protein C Deficient – Depleted Plasma
Protein C Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Protein C Deficient – Depleted Plasma by selective affinity immuno-adsorption using antibodies directed towards Protein C. Available in two different formats – Frozen and Lyophilized
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Our Protein C Deficient – Depleted Plasma can be used for further manufacturing or research use only applications.
Frozen Protein C Deficient Plasma
Product Code: PC-DP
Presentation: Frozen Protein C Deficient – Depleted Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Certificate of Analysis: available upon request
Lyophilized Protein C Deficient Plasma
Product Code: PC-LDP
Presentation: Freeze Dried Protein C Deficient – Depleted Plasma containing 50 mM HEPES and stabilizers.
Reconstitution: Reconstitute with 1 mL of reagent grade water. Allow contents to dissolve for 15 minutes at room temperature with occasional swirling. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Storage and Stability: Prior to reconstitution, dried plasma should be stored at 2-8°C. Product is stable until date stated on vial label when stored at 2-8oC. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Certificate of Analysis: available upon request
Description of Protein C
Protein C is a vitamin K-dependent glycoprotein produced in the liver. The concentration of PC in plasma is ~4 μg/ml (~60 nM). A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis. Protein C is expressed as a two-chain molecule with a molecular weight of 62 kDa. The light chain (21 kDa) of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11 γ-carboxyglutamic acid (gla) residues. These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The heavy chain of PC (41 kDa) consists of the catalytic domain and an activation peptide. Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin. The activation of PC is associated with the release of a small activation peptide (2-3 kDa, called Protein C peptide, or PCP) from the N-terminal of the heavy chain. Activated Protein C (APC) is a serine protease with anticoagulant activity. APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa. The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, also called Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by α1antitrypsin and α2macroglobulin. The inhibitory activity of PCI is stimulated approximately 10 fold by heparin1-3.
References and Reviews
- Broze GJ, Miletich JP; Biochemistry and Physiology of Protein C, Protein S and Thrombomodulin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 259-276, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Esmon CT, Esmon NL, Le Bonniec B, Johnson AE; Protein C Activation; Methods in Enzymology 222, pp 359-385, 1993.
- Heeb MJ, Mosher D, Griffin JH; Activation and Complexation of Protein C and Cleavage and Decrease of Protein S in Plasma of Patients With Intravascular Coagulation; Blood 73, pp 455-461, 1989.
GAPC-IG – Goat anti human Protein C, purified IgG (5mg vial)
SAPC-IG – Sheep anti human Protein C, purified IgG (10mg vial)
SAPC-HRP – Sheep anti human Protein C, peroxidase conjugated IgG (0.2 mg vial)
MAPC-IG – Mouse anti human Protein C, monclonal antibody (0.5mg vial)
SAPC-AP – Sheep anti human Protein C, affinity purified IgG (0.5 mg vial)
PC-EIA – Protein C Paired Antibody Set – 5 plate set
PC-DP – Frozen Protein C Deficient Plasma – 1ml or bulk quantities
PC-LDP – Lyophilized Protein C Deficient Plasma – 1ml
GAPC-AP – Goat anti human Protein C, affinity purified IgG (0.5 mg vial)