Prothrombin Antibodies – Factor II, FII, F2

Affinity Biologicals, Inc. manufactures a broad range of Prothrombin Antibodies – Factor II, FII, F2 which can be found in the listing below.   Further information about each individual Prothrombin Antibodies – Factor II, FII, F2 is available by following the associated links.  Our Prothrombin Antibodies – Factor II, FII, F2 are excellent for us in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays.  These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins.  Affinity’s Prothrombin Antibodies – Factor II, FII, F2 are manufactured for use in research applications.

Listing of Prothrombin Antibodies – Factor II, FII, F2

Description of Prothrombin – Factor II, FII, F2

Prothrombin (factor II, F.II) is a vitamin K-dependent glycoprotein produced in the liver.  The concentration of prothrombin in plasma is ~100 μg/ml (~1.4 μM).  Prothrombin is a single chain molecule with a molecular weight of 72 kDa.  Prothrombin consists of a catalytic domain followed by two kringle structures and an amino-terminal domain containing 10 γ-carboxy-glutamic acid (gla) residues. These gla residues allow prothrombin to bind to membranes that contain acidic phospholipids in a calcium dependent manner.  The binding to membranes is required for effective presentation of prothrombin as a substrate for activation by the prothrombinase complex, which consists of activated factor X, activated cofactor V and calcium on phospholipid membrane.  Activation by prothrombinase occurs by sequential cleavage after residue Arg320 then after Arg271 to produce the active protease α-thrombin (37 kDa) and the by-product prothrombin fragment 1.2 (35 kDa).  The product thrombin further cleaves prothrombin fragment 1.2 after residue Arg155 into individual prothrombin fragments 1 and 2.  The activity of α-thrombin in plasma is inhibited primarily by antithrombin and the rate of inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin.  Other physiological inhibitors of thrombin in the absence of heparin include α2macroglobulin and α1antitrypsin1-3.

References and Reviews

  1. Mann KG; Prothrombin and Thrombin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J
  2. Hirsh, VJ Marder and EW Salzman, pp. 184-199, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
  3. Mann KG; Prothrombin; Methods in Enzymology 45, pp 123-156, 1976.
  4. Downing MW, Bloom JW, Mann KG; Comparison of the Inhibition of Thrombin by Three Plasma Protease Inhibitors; Biochemistry 17, pp 2649-2653, 1978.