Rabbit anti-sheep IgG coated magnetically responsive beads (Isogen Lifescience, Ijsselstein, Netherlands) were reconstituted in coating buffer and incubated with either sheep anti-human thrombin, anti-prothrombin F1, or F2 antibodies (Affinity Biologicals, Ontario, Canada) as per manufacturer recommendations. Remaining IgG binding sites on the magnetically responsive beads were filled by incubating them with non-specific sheep antibodies.
NJ Mutch, T Myles, LLK LeungÃ¢â‚¬Â¦ – Journal of Thrombosis Ã¢â‚¬Â¦, 2010 – Wiley Online Library
… polyclonal anti-prothrombin antibody was from Affinity Biologicals (Ancaster, ON …
CV Murphy, E Eakins, J FaganÃ¢â‚¬Â¦ – Transfusion Ã¢â‚¬Â¦, 2009 – Wiley Online Library
… Prothrombin levels were measured in the diluted samples using
a paired set of antibodies (Affinity Biologicals, Ancaster, ON, Canada), one of which …