Tissue Factor – Published Papers using Affinity Biologicals Products


[PDF] Chimeric Anti-CD14 IGG2/4 Hybrid Antibodies for Therapeutic Intervention in Pig and Human Models of Inflammation

C Lau, KS Gunnarsen, LS Høydahl, JT Andersen… – The Journal of Immunology, 2013
lyzed by flow cytometry. One sample was split into two tubes and stained with sheep anti-human TF (Affinity Biologicals, Ancaster, Canada) and control sheep IgG (Sigma-Aldrich, Saint Louis, MO), respectively. All samples were … 

Combined Inhibition of Complement (C5) and CD14 Markedly Attenuates Inflammation, Thrombogenicity, and Hemodynamic Changes in Porcine Sepsis

A Barratt-Due, EB Thorgersen, K Egge, S Pischke… – The Journal of Immunology, 2013
For measurements of TF on gran- ulocytes, blood was collected at baseline, 0 min, and 180 min, anti- coagulated with citrate, and stained with sheep anti-human TF (Affinity Biologicals, Ancaster, ON, Canada) or control sheep IgG (Sigma-Aldrich, Saint Louis, MO).


Potential factors influencing the development of thrombocytopenia and consumptive coagulopathy after genetically modified pig liver xenotransplantation

B Ekser, CC Lin, C Long, GJ Echeverri, H Hara… – Transplant International, 2012

Flow cytometry to detect TF antigen and microparticles. Baboon platelets and PBMCs were incubated with polyclonal sheep anti-human TF (Affinity Biologicals, Ancaster, ON, Canada) or control sheep IgG (Affinity Biologicals) antibodies for 30 min.


Complement C3 Deficiency Attenuates Chronic Hypoxia-Induced Pulmonary Hypertension in Mice

EM Bauer, H Zheng, S Comhair, S Erzurum… – PLoS ONE, 2011
Recombinant human complement C3a (204881) and recombinant human complement C5a (234397) were from Calbiochem. Tissue factor antibody (SATF-IG) was from Affinity Biologicals. Chronic hypoxia-induced pulmonary hypertension.

Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor-κB

C Ettelaie, D Fountain, MEW Collier, AM ElKeeb… – Biochimica et Biophysica …, 2011
To measure the total cellular TF antigen the cells were lysed in the presence of a protease inhibitor cocktail (Active Motif) and 20 μg of the samples analysed using a TF-antigen ELISA kit (Affinity Biologicals, Ancaster, Canada) as described before [27].

LJ Toltl, RC Austin… – Journal of Thrombosis and …, 2011 – Wiley Online Library
Annexin V–fluorescein isothiocyanate (FITC) was from BD Biosciences (San Jose, CA, USA). Anti-tissue factor (TF)–FITC antibody was from Affinity Biologicals (Ancaster, ON).

LL Swystun, S Mukherjee, M Levine… – Journal of Thrombosis …, 2011 – Wiley Online Library
City). Anti-human TF mAb maTF–fluorescein isothiocyanate (FITC) was from Affinity Biologicals (Ancaster, ON, Canada).

MEW Collier… – Journal of Biological Chemistry, 2011 – ASBMB
Total TF antigen within each isolated sample was measured using a TF EIA (Affinity Biologicals) as described previously (19). Activities of TF-containing and control


JL Yu, R Xing, C Milsom… – Thrombosis Research, 2010 – Elsevier
Briefly, the single cell suspension was incubated with the sheep antibody against human TF (Affinity Biologicals Ancaster, ON, Canada) 

A Maraveyas, C Ettelaie, H Echrish… – Blood Coagulation & …, 2010 – journals.lww.com
serum circulating-tissue factor antigen. The concentration of circulating-tissue factor antigen in the patients’ sera was measured using tissue factor-specific paired antibody ELISA method (Affinity Biologicals, Ancaster, Canada).


LL Swystun, LYY Shin, S Beaudin… – Journal of Thrombosis …, 2009 – Wiley Online Library
Anti-human TF monoclonal antibody SATF was from Affinity Biologicals (Ancaster, ON, Canada), while HTF1 was from BD Pharmigen (San Jose

DE Connor, T Exner, DDF Ma… – Blood Coagulation & …, 2009 – journals.lww.com
Appropriately diluted antibody against human tissue factor (Affinity Biologicals Inc., Hamilton, Canada) was added to samples at concentrations specified in text to reverse the effect of tissue factor.