Antibodies to tPA – Tissue Plasminogen Activator

Tissue Plasminogen Activator (tPA), human

Antibody Host Catalogue No. Size Product Insert
Purified IgG Sheep SATPA-IG 10 mg
Affinity purified IgG Sheep SATPA-AP 0.5 mg
Peroxidase conjugated IgG Sheep SATPA-HRP 0.2 mg
Affinity purified, FITC conjugated IgG Sheep SATPA-APFTC 0.1 mg
Affinity purified, biotinylated IgG Sheep SATPA-APBIO 0.1 mg
Matched Pair Antibodies for EIA TPA-EIA 5 plates

Description of Tissue Plasminogen Activator (tPA)

Tissue-type plasminogen activator (tPA) is one of two major physiologic activators of plasminogen in plasma. It is a serine protease of 68 kDa produced primarily in endothelial cells but is also present in monocytes and megakaryocytes. Normal plasma tPA antigen concentrations have been reported from 20 ng/ml to 5 μg/ml, depending on the assay used, but typically most of the tPA (> 90%) is in complex with it’s primary inhibitor, plasminogen activator inhibitor-1 (PAI-1). Structurally, tPA is a single-chain enzyme that consists of a catalytic domain followed by two kringle structures, an EGF domain and a finger domain. The activation of plasminogen by tPA is dependent on the presence of a fibrin cofactor. The binding of both tPA and plasminogen to fibrin is mediated in part through lysine binding sites within the kringle structures of both enzyme and substrate, but also through the finger domain of tPA. Activation of plasminogen by tPA occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. The activity of tPA is regulated in part by a very short half life in circulation (t½ of ~4 minutes) and by circulating protease inhibitors PAI-1 and to a lesser extent α2macroglobulin1-3.

References:

1. Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.

2. Holvoet P, Cleemput H, Collen D; Assay of Human Tissue-Type Plasminogen Activator (t-PA) with an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to t-PA. Thrombosis and Haemostasis 54, pp. 684-687, 1985.

3. Giles AR, Nesheim, et al; The fibrinolytic Potential of the Normal Primate following the Generation of Thrombin In Vivo; Thrombosis and Haemostasis 63, pp. 476-481, 1990.

Related Products:

SATPA-AP Sheep anti-human tPA, affinity purified IgG

SATPA-HRP Sheep anti-tPA, IgG-peroxidase conjugate

TPA-EIA Paired antibody set for ELISA of tPA, 5 x 96 tests
TPA/PAI human plasma deficient of tPA and PAI-1, frozen