Sheep anti-human Factor VIII
Peroxidase Conjugated IgG
Product #: SAF8C-HRP
Store at –10 to -20oC
For Research Use Only.
Not for use in diagnostic procedures.
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320 kDa) that circulates in plasma at approximately 200 ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of F.VIII ranging in size from 200-280 kDa. The F.VIII is stabilized by association with von Willebrand Factor to form a F.VIII-vWF complex required for the normal survival of F.VIII in vivo (t1/2 of 8-12 hours).
F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, F.VIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay.
Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of F.VIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a F.VIII protein that is partially or totally inactive. The production of neutralizing antibodies to F.VIII also occurs in 5-20% of Hemophiliacs 1-3.
1. Lollar P, Fay PJ, Fass DN; Factor VIII and Factor VIIIa. Methods in Enzymology, 222, pg 122, 1993.
2. Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
3. Pittman DD, Kaufman RJ. Structure-Function Relationships of Factor VIII Elucidated through Recombinant DNA Technology. Thromb. Haemostas. 61:161-165, 1989.
IgG-HRP conjugate as a clear, slightly red-brown liquid.
Human F.VIII (F.VIII:C) purified from concentrate.
absorbance using an extinction coefficient (E1%280) of 14.
A buffered stabilizer solution containing 50% (v/v) glycerol.
Store between –10 and –20oC. Product will become viscous
but will not freeze. Avoid storage in frost-free freezers. Keep
vial tightly capped. Allow product to warm to room
temperature and gently mix before use. Avoid exposure to
sodium azide as this is an inhibitor of peroxidase activity.
Prior to conjugation, this antibody was specific for F.VIII as
demonstrated by immunoelectrophoresis and ELISA. When titrated on vWF-coated plates, the unconjugated SAF8C-IG does not demonstrate any reactivity above the non-immune sheep negative control.
Suitable as a source of peroxidase-labeled antibodies to F.VIII.
Rz Ratio (Reinheitszahl, A403/A280):
Cat #: SAF8C-IG Sheep anti-human Factor VIII, whole IgG from serum
Cat #: SAF8C-AP Sheep anti-human Factor VIII, affinity-purified IgG
Cat #: F8C-EIA Paired antibodies for Factor VIII ELISA, 4 x 96 wells
Cat #: FVIII-DP Human plasma deficient in Factor VIII, immune depleted
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Limited Warranty: This product is warranted to perform in accordance with its labeling and literature. Affinity Biologicals Inc. disclaims any implied warranty of merchantibility or fitness for any other purposes, and in no event will Affinity Biologicals Inc. be liable for any consequential damages arising out of aforesaid express warranty.
Manufactured in Canada by:
AFFINITY BIOLOGICALS INC.
1395 Sandhill Drive
Ancaster ON CANADA L9G 4V5
Tel: (905) 304-9896
Fax: (905) 304-9897