ATIII – Antithrombin Deficient Plasma
Affinity’s ATIII – Antithrombin Deficient Plasma is manufactured from pooled normal human plasma depleted of ATIII – Antithrombin using antibodies directed to ATIII. Only the highest quality citrated plasma is used as starting material. Our Frozen Deficient or Depleted Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and our Lyophilized Deficient Plasma products are typically buffered with the addition of HEPES to 50mM final concentration.
All ATIII – Antithrombin Deficient or Depleted Plasma from Affinity Biologicals are for Research Use Only and must not be used for diagnostic applications.
Antithrombin ATIII Deficient – Depleted Plasma
Antithrombin ATIII Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Antithrombin – ATIII by selective affinity immuno-adsorption using antibodies directed towards both ATIII. Available in two different formats – Frozen and Lyophilized
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Our Antithrombin ATIII Deficient – Depleted Plasma can be used for further manufacturing or research use only applications.
Frozen ATIII Deficient Plasma
Product Code: ATIII-DP
Presentation: Frozen Antithrombin ATIII Deficient – Depleted Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Certificate of Analysis: available upon request
Lyophilized ATIII Deficient Plasma
Product Code: ATIII-LDP
Presentation: Freeze Dried Antithrombin ATIII Deficient – Depleted Plasma containing 50 mM HEPES and stabilizers.
Reconstitution: Reconstitute with 1 mL of reagent grade water. Allow contents to dissolve for 15 minutes at room temperature with occasional swirling. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Storage and Stability: Prior to reconstitution, dried plasma should be stored at 2-8°C. Product is stable until date stated on vial label when stored at 2-8oC. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Certificate of Analysis: available upon request
Description of Antithrombin
Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200 μg/ml (~3.5 μM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59 kDa. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96 kDa1-3.
References and Reviews
- Damus PS, Rosenberg RD; Antithrombin – Heparin Cofactor; Methods in Enzymology 45, pp 653-669, 1976.
- Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.
- Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.
TAT-EIA – Thrombin Antithrombin – TAT Complex Paired Antibody Set – 5 plate set
SAAT-AP – Sheep anti human ATIII – Antithrombin, affinity purified IgG (0.5 mg vial)
SAAT-APBIO – Sheep anti human ATIII – Antithrombin, affinity purified, Biotinylated IgG (0.1 mg vial)
SAAT-IG – Sheep anti human ATIII – Antithrombin, purified IgG (10mg vial)
ATIII-EIA -Antithrombin – ATIII Paired Antibody Set – 5 plate set
SAAT-APHRP – Sheep anti human ATIII – Antithrombin, affinity purified, peroxidase conj. IgG (0.1 mg vial)
GAAT-AP – Goat anti human ATIII – Antithrombin, affinity purified IgG (0.5 mg vial)
GAAT-IG – Goat anti human ATIII – Antithrombin, purified IgG (10mg vial)