Antibody Species Cross Reactivity

Each antibody listed has been tested for reactivity to the plasma of a variety of species. The double immunodiffusion (DID) technique used tests the ability of an antibody to precipitate an antigen in an agarose gel. The DID technique has limitations and a negative result should not necessarily be interpreted as non-recognition of an antigen. A number of antibodies may react with fewer recognition sites on the antigen of another species and be unable to form an insoluble immune complex (this is observed with many monoclonal antibodies). An antibody may demonstrate a greater spectrum of cross reactivity in applications such as immunoblotting and immunohistochemistry where antigen precipitation is not required. Below are examples of typical results seen in historical lots. See product insert for lot specific testing results.

  • Antibodies to: Catalogue # Human Rat Mouse Rabbit Canine Porcine
    Human Fibrinogen SAFG-IG ***
      SAFG-AP *** * * * *
    Rabbit Fibrinogen SARFG-IG *** *** *** *** *** *
      SARFG-AP *** *** *** *** *** *
    Human Prothrombin SAFII-IG *** *
      SAFII-AP *** * * * * *
    Human Thrombin SAHT-IG *** * *
    Rabbit Thrombin SART-IG ***
      SART-AP * * * *** * *
    Bovine Thrombin SABT-IG
    SABT-AP
    Human Factor V SAFV-IG *
    SAFV-AP *
    Human von Willebrand Factor GAVWF-IG *
    GAVWF-AP *
    Human Factor IX SAFIX-IG *
    GAFIX-IG *
    SAFIX-AP *
    GAFIX-AP ***
    Human Factor X GAFX-IG * *
    SAFX-AP *** * *
    GAFX-AP * * *
    Human Factor XI SAFXI-IG *
    GAFXI-IG *
    SAFXI-AP *
    GAFXI-AP *
    Human Factor XII GAFXII-IG *
    GAFXII-AP *** * *
    Human Factor XIII SAFXIII-IG *** * * * *
    Human Factor XIII-A (A Subunit) SAF13A-AP * *
    Human Prekallikrein SAPK-IG **
    SAPK-AP *** * *
    Human Kininogen SAKN-IG ***
    Human Protein C SAPC-IG * *
    GAPC-IG *
    SAPC-AP *
    GAPC-AP *
    Human Protein S SAPS-IG * *
    GAPS-IG *
    SAPS-AP *** * *
    Human Plasminogen SAPG-IG *** *** *** *** *** ***
    GAPG-IG *** *** *** *** *** ***
    SAPG-AP *** *** *** *** *** ***
    GAPG-AP *** *** *** *** *** ***
    Human Antithrombin (ATIII) SAAT-IG ***
    GAAT-IG *** * *
    SAAT-AP *** *** *** * *** ***
    GAAT-AP *** *** *** *** ***
    Human Heparin Co-Factor II SAHC2-IG *** *** *** *** *** *
    GAHC2-IG *** *** *** *** *** ***
    GAHC2-AP *** *** *** *** *** ***
    Human Alpha 2-Antiplasmin SA2AP-IG ***
    GA2AP-IG ***
    GA2AP-AP ***
    Human Alpha 1-Antitrypsin SA1AT-IG ***
    GA1AT-IG ***
    SA1AT-AP ***
    GA1AT-AP ***
    Human Alpha 2-Macroglobulin GAA2M-IG *** *** *** ***
    GAA2M-AP *** * *** *** ***
    Human C1-Inhibitor GACINH-IG ***
    GACINH-AP ***
    Human Beta 2-Glycoprotein-1 (APO-H) GAB2G-IG *** *** *** ***
    GAB2G-AP *** *** * *** ***
    Human Vitronectin SAVN-IG **
      SAVN-AP *** * * * * *
    Legend: *** strong positive * weak positive — negative NT not tested

Matched Pair Antibody Set – Species Cross-Reactivity

The values given are the re-activities relative to normal human reference plasma when the test plasmas are assayed under the conditions specified in the protocol for each assay. Plasmas with reactivities of less than 1% are listed as negative. Optimization of the assay conditions may be required to efficiently utilize these assays for the measurement of these analytes in animal plasmas. Below are examples of typical results seen in historical lots. The only exception is the RBFG-EIA that uses normal rabbit plasma as a reference.

  • Analyte Catalogue No. Human Rat Mouse Rabbit Canine Porcine
    Fibrinogen antigen FG-EIA 100% 17% 7% 8% negative 5%
    Rabbit Fibrinogen antigen RBFG-EIA 40% 5% 7% 100% 18% 4%
    Prothrombin antigen FII-EIA 100% negative negative negative negative negative
    Factor V antigen FV-EIA 100% negative negative 40% negative 26%
    Factor VII antigen FVII-EIA 100% negative negative 8% negative 4%
    Factor VIII antigen F8C-EIA 100% negative negative 18% negative 50%
    von Willebrand Factor antigen VWF-EIA 100% 4% negative 25% 9% 34%
    Factor IX antigen FIX-EIA 100% negative negative 8% negative 7%
    Factor X antigen FX-EIA 100% negative 3% 4% negative 1%
    Factor XI antigen FXI-EIA 100% 5% 4% 77% 6% 12%
    Factor XII antigen FXII-EIA 100% negative negative 5% negative negative
    Kininogen antigen KN-EIA 100% negative negative 4% negative negative
    Prekallikrein antigen PK-EIA 100% negative negative 5% negative negative
    Factor XIII antigen FXIII-EIA 100% negative negative 10% negative negative
    Protein C antigen PC-EIA 100% negative negative negative negative negative
    Protein C Inhibitor antigen PCI-EIA 100% negative negative 6% negative negative
    Protein S antigen PS-EIA 100% negative negative 14% 5% 3%
    Antithrombin antigen ATIII-EIA 100% 14% 23% 7% 11% 4%
    Heparin Cofactor II antigen HCII-EIA 100% 1% 1% 5% 2% negative
    β2-Glycoprotein-1 (Apolipoprotein-H, APO-H) antigen APOH-EIA 100% 7% negative 2% 11% 7%
    Plasminogen antigen PG-EIA 100% negative negative 2% 8% negative

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