Factor IX, human
Factor IX, canine
|Antibody||Host||Catalogue No.||Size||Product Insert|
|Matched Pair Antibodies for EIA||Ã‚Â||CFIX-EIA||4 plates|
Description of Factor IX (FIX)
Factor IX (FIX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FIX is normally around 5 ÃŽÂ¼g/ml (87 nM) in plasma. The biological importance of FIX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity, normal antigen) defect in FIX function.
In its proenzyme or zymogen form FIX is a single chain molecule of 55,000 daltons. It contains two EGF-like domains and an amino-terminal domain containing 12 ÃŽÂ³-carboxy-glutamic acid (Gla) residues. These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146-Ala147 and between Arg180-Val181. The terminal activated product in either case is FIXaÃŽÂ², a two-chain enzyme consisting of a heavy chain (28,000 daltons), a light chain (18,000 daltons) and an activation peptide product of 11,000 daltons. FIX can also be cleaved into inactive products by thrombin and by elastase.
The activity of FIXaÃŽÂ² in plasma is inhibited by antithrombin and this inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin 1-3.
1. Lawson, JH, Mann KG; Cooperative Activation of Human F.IX by the Human Extrinsic Pathway of Coagulation; JBC 266 pp11317-11327, 10991.
2. Enfield DL, Thompson AR; Cleavage and Activation of Factor IX by Serine Proteases; Blood 64, pp 821-831, 1984.
3. Limentani SA, Furie BC, Furie B, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA, 1994
GAFIX-AP Goat anti-human F.IX, affinity purified IgG
GAFIX-HRP Goat anti-human F.IX, IgG-peroxidase
GAFIX-APHRP Goat anti-human F.IX, APIgG-peroxidase
SAFIX-IG Sheep anti-human F.IX, IgG from antiserum
SAFIX-AP Sheep anti-human F.IX, affinity purified IgG
SAFIX-APHRP Sheep anti-human F.IX, APIgG-peroxidase
FIX-EIA Paired antibody set for ELISA of F.IX, 5 x 96 wells
FIX-DP Human plasma deficient in Factor IX, immune depleted
FIX-AG VisuLizeÃ¢â€žÂ¢ FIX Antigen ELISA Kit
INH9-DP Factor IX Inhibitor Plasma, Frozen
INH9-LDP Factor IX Inhibitor Plasma, Lyophilized