Factor V F5 Antibodies

Affinity Biologicals, Inc. manufactures a broad range of Factor V F5 Antibodies to human Factor V which can be found in the listing below.   Further information about each individual Factor V  antibody is available by following the associated links.  Our Factor V F5 Antibodies are excellent for use in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays.  These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins.  Affinity’s Factor V F5 Antibodies are manufactured for use in research applications.

Listing of Factor V F5 Antibodies

Description of Factor V – F5

Factor V – F5 (formerly referred to as accelerator globulin and labile factor) is a large glycoprotein (320 kDa) that is produced in the liver.  The gene that encodes factor V (FV) is located on chromosome 1.  A congenital deficiency of FV is a hemorrhagic disorder inherited as an autosomal recessive disease.

The concentration of FV in plasma is typically 10 μg/mL.  FV is a pro-cofactor that is activated through limited proteolysis by thrombin, or by activated factor X in the presence of phospholipid surface.  Other physiologic activators of FV include plasmin, neutrophil elastase and platelet calpain.  The activated cofactor (FVa) is an essential component of the prothrombin activator complex, which consists of FVa, activated factor X, calcium and anionic phospholipid surface.  The intact prothrombinase complex activates prothrombin to thrombin at a rate 300,000-fold greater than activated factor X alone.  In a positive feedback loop, the thrombin generated accelerates its own generation by activating more FV to FVa.  Thrombin also acts to down-regulate FVa indirectly by activating Protein C, which inactivates FVa cofactor activity1-3.

References and Reviews

  1. Kane WH, Davie EW; Blood Coagulation Factors V and VIII: Structural and functional similarities and their relationship to hemorrhagic and thrombotic disorders.  Blood 71:539, 1988.
  2. Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
  3. Nesheim ME, Katzmann JA, Tracy PB, Mann KG; in Methods in Enzymology 80:249, 1980.