Factor X, human
Factor X, murine
|Antibody||Host||Catalogue No.||Size||Product Insert|
|Matched Pair Antibodies for EIA||Ã‚Â||MFX-EIA||4 plates|
Description of Factor X (FX)
Factor X (FX, Stuart Factor) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of FX in plasma is ~10 ÃŽÂ¼g/ml (~170 nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59 kDa. The light chain (17 kDa) of FX contains a calcium-binding domain consisting of one hydroxy-aspartic acid and 11 ÃŽÂ³-carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of FX (42 kDa) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of FX to the active enzyme (FXa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The FXa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of FXa in plasma is inhibited by antithrombin (ATIII), ÃŽÂ±1antitrypsin, ÃŽÂ±2macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated approximately 1000-fold by heparin1-3.
1. Ichinose A, Davie EW; The Blood Coagulation Factors: Their cDNAs, Genes, and Expression; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 19-54, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
2. Steinberg M, Nemerson Y; The Activation of Factor X; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 91-99, J.B. Lippincott Co., Philadelphia PA, USA, 1982.
3. Ellis V, Scully M, MacGregor I, Kakkar V; Inhibition of Human Factor Xa by Various Plasma Protease Inhibitors; Biochimica et Biophysica Acta 701, pp 24-31, 1982.
GAFX-IG Goat anti-human Factor X, whole IgG from antiserum
GAFX-HRP Goat anti-human Factor X, peroxidase labelled IgG
SAFX-IG Sheep anti-human Factor X, IgG from antiserum
SAFX-AP Sheep anti-human Factor X, affinity purified IgG
RAFX-HRP Rabbit anti-human Factor X, peroxidase labelled IgG
FX-EIA Paired antibody set for ELISA of Factor X, 5 x 96 wells
FX-DP Human plasma deficient in Factor X, immune depleted
INH10-DP Factor X Inhibitor Plasma, Frozen
INH10-LDP Factor X Inhibitor Plasma, Lyophilized