Antibodies to Factor X

Factor X, human

Antibody Host Catalogue No. Size Product Insert
Purified IgG Goat GAFX-IG 10 mg
Affinity purified IgG Goat GAFX-AP 0.5 mg
Peroxidase conjugated IgG Goat GAFX-HRP 0.2 mg
Affinity purified IgG Sheep SAFX-AP 0.5 mg
Affinity purified, FITC conjugated IgG Sheep SAFX-APFTC 0.1 mg
Affinity purified, biotinylated IgG Sheep SAFX-APBIO 0.1 mg
Peroxidase conjugated IgG Rabbit RAFX-HRP 0.2 mg
Matched Pair Antibodies for EIA   FX-EIA 5 plates

Factor X, murine

Antibody Host Catalogue No. Size Product Insert
Matched Pair Antibodies for EIA   MFX-EIA 4 plates

 

Description of Factor X (FX)

Factor X (FX, Stuart Factor) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of FX in plasma is ~10 μg/ml (~170 nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59 kDa. The light chain (17 kDa) of FX contains a calcium-binding domain consisting of one hydroxy-aspartic acid and 11 γ-carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of FX (42 kDa) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of FX to the active enzyme (FXa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The FXa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of FXa in plasma is inhibited by antithrombin (ATIII), α1antitrypsin, α2macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated approximately 1000-fold by heparin1-3.

References:

1. Ichinose A, Davie EW; The Blood Coagulation Factors: Their cDNAs, Genes, and Expression; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 19-54, J.B. Lippincott Co., Philadelphia PA, USA, 1994.

2. Steinberg M, Nemerson Y; The Activation of Factor X; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 91-99, J.B. Lippincott Co., Philadelphia PA, USA, 1982.

3. Ellis V, Scully M, MacGregor I, Kakkar V; Inhibition of Human Factor Xa by Various Plasma Protease Inhibitors; Biochimica et Biophysica Acta 701, pp 24-31, 1982.

Related Products:

GAFX-IG Goat anti-human Factor X, whole IgG from antiserum

GAFX-HRP Goat anti-human Factor X, peroxidase labelled IgG

SAFX-IG Sheep anti-human Factor X, IgG from antiserum

SAFX-AP Sheep anti-human Factor X, affinity purified IgG

RAFX-HRP Rabbit anti-human Factor X, peroxidase labelled IgG

FX-EIA Paired antibody set for ELISA of Factor X, 5 x 96 wells

FX-DP Human plasma deficient in Factor X, immune depleted

INH10-DP Factor X Inhibitor Plasma, Frozen

INH10-LDP Factor X Inhibitor Plasma, Lyophilized