Factor XIII F13 Antibodies
Affinity Biologicals, Inc. manufactures a broad range of Factor XIII F13 Antibodies which can be found in the listing below. Further information about each individual Factor XIII antibody is available by following the associated links. Our Factor XIII F13 Antibodies are excellent for use in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays. These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins. Affinity’s Factor XIII F13 Antibodies are manufactured for use in research applications.
Listing of Factor XIII F13 Antibodies
SAF13A-IG – Sheep anti human Factor XIII (A-subunit), purified IgG (10mg vial)
SAFXIII-IG – Sheep anti human Factor XIII, purified IgG (10mg vial)
SAFXIII-HRP – Sheep anti human Factor XIII, peroxidase conjugated IgG (0.2 mg vial)
SAF13A-APBIO – Sheep anti human Factor XIII (A subunit), affinity purified, Biotinylated IgG (0.1 mg vial)
SAF13A-APFTC – Sheep anti human Factor XIII (A subunit), affinity purified, FITC conjugated IgG (0.1 mg vial)
SAF13A-HRP – Sheep anti human Factor XIII (A subunit), peroxidase conjugated IgG (0.2 mg vial)
SAF13A-AP – Sheep anti human Factor XIII (A-subunit), affinity purified IgG (0.5 mg vial)
FXIII-EIA – Factor XIII Paired Antibody Set – 5 plate set
Description of Factor XIII F13
Factor XIII (FXIII, fibrin stabilizing factor) is the proenzyme form of a transamidase that is essential for normal haemostasis and fibrinolysis, wound healing, female fertility and foetal development. Extracellular F.XIII consists of A subunits (83 kDa each) which contain the enzyme moiety, and B subunits (76 kDa each) which act as a carrier protein for the A subunit in circulation. Both subunits are produced under separate genetic control. In plasma, FXIII exists as a non-covalent tetrameric complex (320 kDa) of two A-subunits and two B-subunits (A2B2). The concentration of F.XIII tetramer in plasma is ~25 μg/ml (~80 nM). An intracellular form of FXIII is found in platelets, megakaryocytes and monocytes. This form of F.XIII presents as a dimer of two A-subunits only and has a molecular weight of 160 kDa. The importance of these intracellular stores is demonstrated by the observation that platelets can contribute up to half of the FXIII activity in platelet rich plasma. The activation of FXIII involves several steps. Thrombin cleaves after Arg37 of each A-subunit in the A2B2 tetramer, releasing a 4.5 kDa activation peptide. Additional conformational changes induced by the binding of calcium, and by dissociation of the B-subunits from the A-subunit dimer are required to obtain full enzyme activity. FXIIIa is a cysteine protease that catalyses the formation of γ-glutamyl-ε-lysyl bonds between the γ and α chains of polymerised fibrin molecules. Other proteins found crosslinked into fibrin clots by FXIIIa include fibrinogen, α2antiplasmin, fibronectin, vitronectin and von Willebrand factor 1-3.
References and Reviews
- McDonagh J; Structure and Function of Factor XIII; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 301-313, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Inbal A, Muszbek L; Coagulation Factor Deficiencies and Pregnancy Loss; Seminars in Thrombosis and Haemostasis 29, pp 171-174, 2003.
- Murdock PJ, Owens DL, Chitolie A, Hutton RA, Lee CA; Development and Evaluation of ELISAs for Factor XIIIA and XIIIB Subunits in Plasma; Thrombosis Research 67, pp 73-79, 1992.