Plasminogen Deficient Plasma - Affinity Biologicals, Inc.

Plasminogen Deficient – Depleted Plasma

Plasminogen Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Plasminogen by selective affinity immuno-adsorption using antibodies directed towards Plasminogen. Available in two different formats – Frozen and Lyophilized

Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Our Plasminogen Deficient – Depleted Plasma can be used for further manufacturing or research use only applications.

Plasminogen Deficient – Depleted Plasma

Plasminogen Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Plasminogen Deficient – Depleted Plasma by selective affinity immuno-adsorption using antibodies directed towards PG. Available in two different formats – Frozen and Lyophilized

Frozen Plasminogen Deficient Plasma

Product Code: PK-DP

Presentation: Frozen Plasminogen Deficient – Depleted Plasma

Preparation/Handling: Thaw 1 ml vials in 37^oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.

Storage and Stability: Plasma is shipped frozen and should be stored below -60^oC. Product is stable until date stated on vial label when stored at -60^oC. Once thawed, plasma is stable for 4 hours at 2-8^oC in original vial.

Certificate of Analysis: available upon request

Lyophilized Plasminogen Deficient Plasma

Product Code: PG-LDP

Presentation: Freeze Dried Plasminogen Deficient – Depleted Plasma containing 50 mM HEPES and stabilizers.

Reconstitution: Reconstitute with 1 mL of reagent grade water. Allow contents to dissolve for 15 minutes at room temperature with occasional swirling. Stability after reconstitution is 4 hours at ambient (18-25^oC), or 30 days at –20^oC.

Storage and Stability: Prior to reconstitution, dried plasma should be stored at 2-8°C. Product is stable until date stated on vial label when stored at 2-8^oC. Stability after reconstitution is 4 hours at ambient (18-25^oC), or 30 days at –20^oC.

Certificate of Analysis: available upon request

Description of Plasminogen

Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 μg/ml (~2.3 μM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to α2antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is α2antiplasmin. Other physiological inhibitors of plasmin include α2macroglobulin and antithrombin^1-3.

References and Reviews

Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3^rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
Castellino FJ, Powell JR; Human Plasminogen; Methods in Enzymology 80, pp 365-378, 1981.
Wiman B, Collen D; Molecular Mechanism of Physiological Fibrinolysis; Nature 272, pp 548-553, 1978.

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