Antibodies to Plasminogen

Antibodies to Plasminogen, human

Antibody Host Catalogue No. Size Product Insert
Purified IgG Goat GAPG-IG 5 mg
Affinity purified IgG Goat GAPG-AP 0.5 mg
Affinity purified, peroxidase conj. IgG Goat GAPG-APHRP 0.1 mg
Purified IgG Sheep SAPG-IG 5 mg
Affinity purified IgG Sheep SAPG-AP 0.5 mg
Matched Pair Antibodies for EIA   PG-EIA 5 plates
Analyte Specific Reagent
– Affinity purified IgG Goat GAPG-AP-ASR 0.5 mg

Antibodies to Plasminogen, murine

Antibody Host Catalogue No. Size Product Insert
Matched Pair Antibodies for EIA   MPG-EIA 4 plates

Description of Plasminogen (Pg)

Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 μg/ml (~2.3 μM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to α2antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is α2antiplasmin. Other physiological inhibitors of plasmin include α2macroglobulin and antithrombin1-3.

References:

1. Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.

2. Castellino FJ, Powell JR; Human Plasminogen; Methods in Enzymology 80, pp 365-378, 1981.

3. Wiman B, Collen D; Molecular Mechanism of Physiological Fibrinolysis; Nature 272, pp 548-553, 1978.

Related Products:

GAPG-IG Goat anti-human plasminogen, IgG from antiserum

GAPG-AP Goat anti-human plasminogen, affinity purified IgG

GAPG-APHRP Goat anti-plasminogen, APIgG-peroxidase conjugate

SAPG-AP Sheep anti-human plasminogen, affinity purified IgG

PG-EIA Paired antibody set for ELISA of Pg, 5 x 96 tests
PG-DP Human plasma deficient in Plasminogen (Pg), immune depleted, Frozen
PG-LDP Human plasma deficient in Plasminogen (Pg), immune depleted, Lyophilized