Affinity Biologicals, Inc. manufactures a broad range of Plasminogen Antibodies which can be found in the listing below. Further information about each individual Plasminogen Antibody is available by following the associated links. Our Plasminogen Antibodies are excellent for use in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays. These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins. Affinity’s Plasminogen Antibodies are manufactured for use in research applications.
Listing of Plasminogen Antibodies
GAPG-IG – Goat anti human Plasminogen, purified IgG (5mg vial)
SAPG-IG – Sheep anti-human Plasminogen, purified IgG (5mg vial)
GAPG-APHRP – Goat anti human Plasminogen, affinity purified, peroxidase conj. IgG (0.1 mg vial)
GAPG-AP – Goat anti human Plasminogen, affinity purified IgG (0.5 mg vial)
SAPG-AP – Sheep anti human Plasminogen, affinity purified IgG (0.5 mg vial)
MPG-EIA – Murine Plasminogen Paired Antibody Set – 4 plate set
PG-EIA – Plasminogen Paired Antibody Set – 5 plate set
Description of Plasminogen – PLG
Plasminogen – PLG is synthesized in the liver and circulates in plasma at a concentration of ~200 μg/ml (~2.3 μM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to α2antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is α2antiplasmin. Other physiological inhibitors of plasmin include α2macroglobulin and antithrombin1-3.
References and Review
- Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Castellino FJ, Powell JR; Human Plasminogen; Methods in Enzymology 80, pp 365-378, 1981.
- Wiman B, Collen D; Molecular Mechanism of Physiological Fibrinolysis; Nature 272, pp 548-553, 1978.