Antibodies to Plasminogen, human
Antibodies to Plasminogen, murine
|Antibody||Host||Catalogue No.||Size||Product Insert|
|Matched Pair Antibodies for EIA||Â||MPG-EIA||4 plates|
Description of Plasminogen (Pg)
Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 Î¼g/ml (~2.3 Î¼M). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to Î±2antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is Î±2antiplasmin. Other physiological inhibitors of plasmin include Î±2macroglobulin and antithrombin1-3.
1. Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
2. Castellino FJ, Powell JR; Human Plasminogen; Methods in Enzymology 80, pp 365-378, 1981.
3. Wiman B, Collen D; Molecular Mechanism of Physiological Fibrinolysis; Nature 272, pp 548-553, 1978.
GAPG-IG Goat anti-human plasminogen, IgG from antiserum
GAPG-AP Goat anti-human plasminogen, affinity purified IgG
GAPG-APHRP Goat anti-plasminogen, APIgG-peroxidase conjugate
SAPG-AP Sheep anti-human plasminogen, affinity purified IgG
PG-EIA Paired antibody set for ELISA of Pg, 5 x 96 tests
PG-DP Human plasma deficient in Plasminogen (Pg), immune depleted, Frozen
PG-LDP Human plasma deficient in Plasminogen (Pg), immune depleted, Lyophilized