beta-2-glycoprotein-1 Polyclonal Antibody
Affinity’s beta-2-glycoprotein-1 Polyclonal Antibody – formerly known as Apoliprotein-H (APOH) is the base level of our beta-2-glycoprotein-1 antibody family. The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum. This beta-2-glycoprotein-1 Polyclonal Antibody is generally intended for use in applications such as immuno-precipitation, immuno-electrophoresis, immuno-depletion and activity neutralization assays.
Product Code: GAB2G-IG
Retail Product Size: 5mg vial
Host Animal: Goat Anti-Human beta-2-glycoprotein-1 Polyclonal Antibody
Species Cross Reactivity: View Chart
Product Datasheet: Beta 2 glycoprotein B2GP1 Polyclonal Antibody, purified anti-human goat IgG
Description of Beta-2-Glycoprotein-1 (Apolipoprotein-H, APOH)
Beta-2-Glycoprotein 1 (B2GP1), also known as Apolipoprotein-H (APOH), is a plasma glycoprotein that circulates at a concentration of 200 ug/ml (4 μM). Synthesized in the liver, β2GP1 is a single chain molecule of 48 kDa, consisting of five repeating internally disulphide-bonded structures referred to as sushi domains. Relative to other glycoproteins, β2GP1 has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%). Almost half the circulating β2GP1 in plasma is associated with lipoproteins of all major fractions. β2GP1 has been demonstrated to bind negatively charged phospholipids, heparin and platelets. Although the precise function(s) are as yet unknown, β2GP1 has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage. Recent evidence also implicates β2GP1 as a cofactor recognized by anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE)1-3.
References and Review
- Lee, NS, Brewer HB Jr., Osborne JC Jr.; β2Glycoprotein I: Molecular Properties of an Unusual Apolipoprotein, Apolipoprotein H; JBC 258, pp 4765-4770, 1983.
- Schousboe I; β2Glycoprotein I: A Plasma Inhibitor of the Contact Activation of the Intrinsic Blood Coagulation Pathway. Blood 66, pp 1086-1091, 1985.
- Nimpf J, Bevers EM, Bomans PHH, Till U, Wurm H, Kostner GM, Zwaal RFA; Prothrombinase activity of human platelets is inhibited by β2Glycoprotein I; Biochimica et Biophysica Acta 884, pp 142-149, 1986.