Affinity Biologicals, Inc.
  • HOME
  • ABOUT US
    • Upcoming Meetings
  • PRODUCTS
    • Antibodies
    • Matched-Pair Antibody Sets
    • Complete ELISA Kits
      • Factor VII ELISA Kit
      • Factor VIII ELISA Kit
      • Factor IX ELISA Kit
      • Factor X ELISA Kit
      • Factor XI ELISA Kit
      • TAFI ELISA Kit
    • Deficient Plasmas
      • Frozen – Research
      • Lyophilized – Research
      • Frozen – Diagnostic
    • Control Plasma – Diagnostic
      • Frozen Calibrator – Reference Plasma
      • Frozen Normal Controls
      • Frozen Abnormal Controls
      • Multi-Level Controls
      • Low Fibrinogen Control Plasma
    • Factor Inhibitor Plasmas
    • Normal Donor Plasma Set
    • Custom Human Plasma
  • SERVICES
    • Custom Polyclonal Antibody Production
    • Contract Manufacturing
    • Specialty Disease State Plasmas & Hemostasis Controls
    • Custom Protein Purification
  • TECHNICAL
    • FAQ – Frequently Asked Questions
    • ELISA and Immunoblotting Methods
    • Species Cross Reactivity
    • Polyclonal Antibody Formats
    • Antibody Applications
    • Antibody Concentration Ranges
    • Antibody Storage – Handling Recommendations
    • Safety Data Sheets – SDS
  • ORDERING
    • How to Order
    • Find a Distributor
    • Quote Request Form
  • CONTACT
    • How to Find Us
    • Contact Form
Your cart is empty. Go to Shop.
Home / Product Family / Factor IX Products / Factor IX Polyclonal Antibody – Affinity Purified – Goat
Fibrinogen, Prothrombin, Thrombin, Factor II, V, VII, VIII, IX, X, XI, XII, XII, XIII, Tissue Factor, vWF, PK, KN, Protein C, S, Z, Plasminogen, tPA, TAFI, ATIII, HCII, PAI-1, A2AP, PCI, A1AT, A2M, C1 INH, Elastase, B2GP1, APOH, Vitronectin, Vimentin, Platelet Factor 4, Platelet glycoprotein 2b/3A

Factor IX Polyclonal Antibody – Affinity Purified – Goat

GAFIX-AP – Goat anti human Factor IX, affinity purified IgG (0.5 mg vial)

SKU: GAFIX-AP Categories: Antibodies, Factor IX Products, Factor IX Antibody Tags: F9, Factor IX, polyclonal antibody, Products, Antibodies

Description

Factor IX Polyclonal Antibody – Affinity Purified – Goat

Affinity’s Factor IX Polyclonal Antibody – Affinity Purified – Goat is the highest level of our Factor IX antibody family.  During the Antigen Affinity Purification process the IgG has had any non-specific immunoglobulin fraction eliminated which enriches the specificity of the remaining immunoglobulin towards the target antigen.  The result is a very high-purity product with a substantially higher titre than whole or purified IgG.  Our Factor IX Polyclonal Antibody – Affinity Purified – Goat is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v) and is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.


Product Code: GAFIX-AP

Retail Product Size: 0.5mg vial

Host Animal: Goat Anti-Human Factor IX Polyclonal Antibody – Affinity Purified

Species Cross Reactivity: View Chart

Product Datasheet: Factor IX F9 Polyclonal Antibody, affinity purified anti-human sheep IgG


Description of Factor IX (FIX)

Factor IX (FIX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FIX is normally around 5 μg/ml (87 nM) in plasma. The biological importance of FIX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity, normal antigen) defect in FIX function.

In its proenzyme or zymogen form FIX is a single chain molecule of 55,000 daltons. It contains two EGF-like domains and an amino-terminal domain containing 12 γ-carboxy-glutamic acid (Gla) residues. These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146-Ala147 and between Arg180-Val181. The terminal activated product in either case is FIXaβ, a two-chain enzyme consisting of a heavy chain (28,000 daltons), a light chain (18,000 daltons) and an activation peptide product of 11,000 daltons. FIX can also be cleaved into inactive products by thrombin and by elastase.

The activity of FIXaβ in plasma is inhibited by antithrombin and this inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin 1-3.

References and Reviews

  1. Lawson, JH, Mann KG; Cooperative Activation of Human F.IX by the Human Extrinsic Pathway of Coagulation; JBC 266 pp11317-11327, 10991.
  2. Enfield DL, Thompson AR; Cleavage and Activation of Factor IX by Serine Proteases; Blood 64, pp 821-831, 1984.
  3. Limentani SA, Furie BC, Furie B, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA, 1994

Keyword Search

Filter Products

show blocks helper
Show products filter form
Affinity Biologicals, Inc.
Affinity Biologicals, Inc. © 2019
+1-905-304-9896
+1-800-903-6020Can/US
info@affinitybiologicals.com
1348 Sandhill Drive
Ancaster, ON
L9G 4V5

Privacy Statement
Sitemap
Terms and Conditions