Factor V Deficient Plasma

Description

Factor V Deficient Plasma is manufactured from normal citrated human plasma that has been depleted of Factor V by selective affinity immuno-adsorption using antibodies directed towards FV.

Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and are available in 1ml vials to litre quantities. Our Factor V deficient plasma can be used for further manufacturing or research use only applications.

Product Code: FV-DP

Presentation: Frozen Factor V Deficient Plasma

Preparation/Handling: Thaw 1 ml vials in 37^oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.

Storage and Stability: Plasma is shipped frozen and should be stored below -60^oC. Product is stable until date stated on vial label when stored at -60^oC. Once thawed, plasma is stable for 4 hours at 2-8^oC in original vial.

Certificate of Analysis: available upon request

Description of Factor V

Factor V (formerly referred to as accelerator globulin and labile factor) is a large glycoprotein (320 kDa) that is produced in the liver. The gene that encodes factor V (FV) is located on chromosome 1. A congenital deficiency of FV is a hemorrhagic disorder inherited as an autosomal recessive disease. The concentration of FV in plasma is typically 10 μg/mL. FV is a pro-cofactor that is activated through limited proteolysis by thrombin, or by activated factor X in the presence of phospholipid surface. Other physiologic activators of FV include plasmin, neutrophil elastase and platelet calpain. The activated cofactor (FVa) is an essential component of the prothrombin activator complex, which consists of FVa, activated factor X, calcium and anionic phospholipid surface. The intact prothrombinase complex activates prothrombin to thrombin at a rate 300,000-fold greater than activated factor X alone. In a positive feedback loop, the thrombin generated accelerates its own generation by activating more FV to FVa. Thrombin also acts to down-regulate FVa indirectly by activating Protein C, which inactivates FVa cofactor activity^1-3.

References and Reviews

Kane WH, Davie EW; Blood Coagulation Factors V and VIII: Structural and functional similarities and their relationship to hemorrhagic and thrombotic disorders. Blood 71:539, 1988.
Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3^rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
Nesheim ME, Katzmann JA, Tracy PB, Mann KG; in Methods in Enzymology 80:249, 1980.

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