Heparin Cofactor II Polyclonal Antibody – Affinity Purified – HRP Conjugated
Affinity’s Heparin Cofactor II Polyclonal Antibody – Affinity Purified – HRP Conjugated is the highest level of our horseradish peroxidase conjugated Heparin Cofactor II antibodies. During the Antigen Affinity Purification process the IgG has had any non-specific immunoglobulin fraction eliminated which enriches the specificity of the remaining immunoglobulin towards the target antigen. The result is a very high-purity product with a substantially higher titre than whole or purified IgG. Our Heparin Cofactor II Polyclonal Antibody – Affinity Purified – HRP Conjugated is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v) and has been conjugated with Horseradish Peroxidase as an enzyme reporter. This antibody is generally intended for use as labeled primary antibodies in applications such as immunoassay and immunoblotting.
Product Code: GAHC2-APHRP
Retail Product Size: 0.1mg vial
Host Animal: Goat Anti-Human Heparin Cofactor II Polyclonal Antibody – Affinity Purified – HRP Conjugated
Species Cross Reactivity: View Chart
Description of Heparin CoFactor II
Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 μg/ml (0.8-1.5 μM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.
References and Reviews
- Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.
- Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.
- Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.