Plasminogen Paired Antibody Set
Affinity’s Plasminogen Paired Antibody Set consists of matched capture and detecting antibodies only that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for five full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This Plasminogen Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications. Assay validation is the responsibility of the end user.
Product Code: PG-EIA
- Capture Antibody (PG-EIA-C): One yellow-capped vial containing 0.4 ml of polyclonal affinity purified anti-Plasminogen antibody for coating plates.
- Detecting Antibody (PG-EIA-D): One red-capped tube containing 0.4 ml of peroxidase conjugated affinity-purified polyclonal anti-Plasminogen antibody for detection of captured Plasminogen.
Species Cross Reactivity: View Chart
Product Datasheet: PG Plasminogen Antigen Matched Pair Antibody Set for ELISA - PG-EIA
Description of Plasminogen
Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 μg/ml (~2.3 μM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to α2antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is α2antiplasmin. Other physiological inhibitors of plasmin include α2macroglobulin and antithrombin1-3.
References and Review
- Bachmann F; The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Castellino FJ, Powell JR; Human Plasminogen; Methods in Enzymology 80, pp 365-378, 1981.
- Wiman B, Collen D; Molecular Mechanism of Physiological Fibrinolysis; Nature 272, pp 548-553, 1978.