Menu
Affinity Biologicals, Inc.
  • HOME
  • ABOUT US
    • Upcoming Meetings
  • PRODUCTS
    • Antibodies
    • Matched-Pair Antibody Sets
    • Complete ELISA Kits
      • Factor VII ELISA Kit
      • Factor VIII ELISA Kit
      • Factor VIII PLUS ELISA Kit
      • Factor IX ELISA Kit
      • Factor X ELISA Kit
      • Factor XI ELISA Kit
      • TAFI ELISA Kit
    • Deficient Plasmas
      • Frozen – Research
      • Lyophilized – Research
      • Frozen – Diagnostic
    • Control Plasma – Diagnostic
      • Frozen Calibrator – Reference Plasma
      • Frozen Normal Controls
      • Frozen Abnormal Controls
      • Multi-Level Controls
      • Low Fibrinogen Control Plasma
    • Factor Inhibitor Plasmas
    • Normal Donor Plasma Set
    • Custom Human Plasma
  • SERVICES
    • Custom Polyclonal Antibody Production
    • Contract Manufacturing
    • Specialty Disease State Plasmas & Hemostasis Controls
    • Custom Protein Purification
  • TECHNICAL
    • FAQ – Frequently Asked Questions
    • ELISA and Immunoblotting Methods
    • Species Cross Reactivity
    • Polyclonal Antibody Formats
    • Antibody Applications
    • Antibody Concentration Ranges
    • Antibody Storage – Handling Recommendations
  • SDS – Product Safety Data Sheets
  • ORDERING
    • How to Order
    • Find a Distributor
    • Quote Request Form
  • CONTACT
    • How to Find Us
    • Contact Form
Close Menu
Home / Paired Antibody Sets / Prekallikrein EIA / Prekallikrein Paired Antibody Set
Prekallikrein Paired Antibody Set

Prekallikrein Paired Antibody Set

PK-EIA – Prekallikrein Paired Antibody Set – 4 plate set

SKU: PK-EIA Categories: Antibodies, Paired Antibody Sets, Prekallikrein Antibody, Prekallikrein Products, Prekallikrein EIA Tags: Prekallikrein, Products, Antibodies, Prekallikrein Paired Antibody Set

Description

Prekallikrein Paired Antibody Set

Affinity’s Prekallikrein Paired Antibody Set consists of matched capture and detecting antibodies that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for four full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This Prekallikrein Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications.  Assay validation is the responsibility of the end user.


Product Code: PK-EIA

Supplied Materials:

  1. Capture Antibody (PK-EIA-C): One yellow-capped vial containing 0.4 ml of polyclonal affinity purified anti-Prekallikrein antibody for coating plates.
  2. Detecting Antibody (PK-EIA-D):  One red-capped tube containing 0.4 ml of peroxidase conjugated affinity-purified polyclonal anti-Prekallikrein antibody for detection of captured Prekallikrein.

Related Products:  VisuLize Buffer Pak  Prekallikrein Deficient Plasma

Species Cross Reactivity: View Chart

Product Datasheet: Prekallikrein Fletcher Factor PK Matched Pair Antibody Set for ELISA


Description of Prekallikrein (PK)

Prekallikrein (PK), previously known as Fletcher Factor, is a single chain glycoprotein produced in the liver. The plasma concentration of PK is 50 μg/ml (550 nM), approximately 75% of which circulates in complex with high molecular weight kininogen (HK) and the remainder as free PK. Plasma PK is heterogeneous in both mass and charge due to variable degrees of glycosylation. Approximately 90% of plasma PK has an apparent molecular weight of 88 kDa as determined by SDS-PAGE and the remaining 10% has an apparent mass of 85 kDa. The catalytic site resides in the light chain. The heavy chain of PK contains four apple-domain structures similar to those found in FXI and these are required for binding of PK to HK. PK is the zymogen form of the enzyme kallikrein, which is involved in the proteolysis of kininogens with subsequent release of bradykinin, a potent vasodilator. PK participates in the contact phase of coagulation as a substrate for surface-bound activated factor XII (FXIIa) in the presence of the cofactor HK. As PK and factor XI (FXI) both circulate in complex with HK, both are localized to activating surfaces through their respective binding to HK. Limited proteolysis of PK by FXIIa generates kallikrein, a two-chain serine protease that initiates the reciprocal activation of PK and FXI. Kallikrein activity in plasma is regulated predominantly by C1-Inhibitor and α2macroglobulin, with relatively minor contributions by Protein C Inhibitor, α2antiplasmin, and antithrombin1-3.

References and Reviews:

  1. Coleman RW, Schmaier AH; Contact System: A Vascular Biology Modulator With Anticoagulant, Profibrinolytic, Antiadhesive and Proinflammatory Attributes. Blood 90, pp 3819-3843, 1997.
  2. DeLa Cadena R, Watchtfogel YT, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 219-240, J.B. Lippincott Co., Philadelphia, 1994.
  3. Hojima Y, Pierce JV, Pisano JJ; Purification and Characterization of Multiple Forms of Human Plasma Prekallikrein. JBC 260, pp 400-406, 1985.

 

Keyword Search

Filter Products

show blocks helper
Show products filter form
Affinity Biologicals, Inc.
+1-905-304-9896
+1-800-903-6020Can/US
info@affinitybiologicals.com
1348 Sandhill Drive
Ancaster, ON
L9G 4V5

Privacy Statement
Sitemap
Terms and Conditions