vWF – von Willebrand Factor Polyclonal Antibody
Affinity’s vWF – von Willebrand Factor Polyclonal Antibody is the base level of our vWF – von Willebrand Factor antibody family. The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum. This vWF – von Willebrand Factor Polyclonal Antibody is generally intended for use in applications such as immuno-precipitation, immuno-electrophoresis, immuno-depletion and activity neutralization assays.
Product Code: GAVWF-IG
Retail Product Size: 2.5mg vial
Host Animal: Goat Anti-Human vWF – von Willebrand Factor Polyclonal Antibody
Species Cross Reactivity: View Chart
Description of von Willebrand Factor (vWF)
von Willebrand Factor (vWF, also previously referred to as Factor VIII related antigen) is a large adhesive protein produced in endothelial cells and megakaryocytes. There are two critical functions of vWF, the first being its involvement in the process of platelet adhesion and aggregation through interaction with platelet receptor glycoprotein Ib, the second being the binding and stabilization of Factor VIII (antihemophilic factor) for secretion and transport in plasma. The vWF precursor protein is synthesized with a 95,000 dalton propeptide (also known as vWF antigen-II), believed to be involved in the intracellular multimerization of the vWF subunits. The mature vWF multimers are then packed into storage organelles within the cell (Weibel-Palade bodies) after which the propeptide is cleaved and released. vWF circulates as multimers of disulphide linked 220,000 dalton subunits and the molecular weight of these multimers ranges from 0.5-20 million daltons.
The plasma concentration of vWF is typically 10 μg/ml, but increased levels are often observed in pregnancy and other conditions of physiological stress. von Willebrand’s disease (vWD) is perhaps the most common inherited bleeding disorder in humans and is the result of either quantitative deficiencies of vWF (vWD Types I & III), or one of a number of qualitative disorders of vWF structure and function (vWD Type II).
References and Reviews
- Montgomery RR, Coller BS, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 134-168, J.B. Lippincott Co., Philadelphia PA,.USA, 1994.
- Sadler JE; von Willebrand Factor (Minireview); JBC 266:34, pp 22777-22780, 1991.