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Factor VIII Antigen Plus Kit

ELISA Kits

The VisuLize™ Factor VIII Antigen Plus Kit is an Enzyme Immunoassay for the quantitative determination of human Factor VIII (FVIII) antigen in plasma samples and FVIII concentrates using the double antibody enzyme linked immuno-sorbent assay (ELISA).

Features

  • Rapid sandwich ELISA to measure human FVIII antigen in plasma samples and FVIII concentrates (FVIII:Ag)
  • Detection limit of 0.008 IU/mL (0.8 %) FVIII antigen
  • Under 3 hour total incubation time
  • FVIII antigen reported as IU/mL determined by comparison to a secondary standard that is traceable to the WHO International Standard for FVIII antigen
  • Includes normal and low controls
  • Mean lot-to-lot variability is 3.1%

Specifications

Storage and Shelf Life

Storing

between 2°C and 8°C

Type

Liquid

Type

Lyophilized

Expiration

Please inquire

Available Formats

Cat # Format
F8PLUS-AG

Pre-coated 96-well plate; 6x16 strip format

2 x Calibrator Plasma

2 x Control Plasma A

2 x Control Plasma B

1 x 50.0 mL 20X Wash Buffer Concentrate

3 x 20.0 mL Buffered Sample Diluent

1 x 12.0 mL Detecting Antibody

1 x 12.0 mL TMB Substrate

1 x 12.0 mL Stop Solution

For Research Use Only.

Comparative Recovery of FVIII:Ag with Various FVIII Therapeutic Concentrates

Presented at ISTH 2021

In this study, we assessed the FVIII recognition of various FVIII therapeutic products available, including those with PEG and dimeric Fc modifications, in both the 1st and 2nd generation FVIII antigen kits.

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