Protein C Antibodies
Affinity Biologicals, Inc. manufactures a broad range of Protein C Antibodies which can be found in the listing below. Further information about each individual Protein C antibody is available by following the associated links. Our Protein C Antibodies are excellent for use in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays. These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins. Affinity’s Protein C Antibodies are manufactured for use in research applications.
|Protein C, Human||Host||Catalogue No.||Size|
|Purified IgG||Goat||GAPC-IG||5mg vial|
|Affinity Purified IgG||Goat||GAPC-AP||0.5mg vial|
|Purified IgG||Sheep||SAPC-IG||10mg vial|
|Affinity Purified IgG||Sheep||SAPC-AP||0.5mg vial|
|Peroxidase Conjugated IgG||Sheep||SAPC-HRP||0.2mg vial|
|For EIA of Protein C||PC-EIA||5 plates|
|For EIA of APC-PCI Complex||APCPCI-EIA||5 plates|
|Protein C, Murine||Host||Catalogue No.||Size|
|Murine Monoclonal, IgG||Mouse||MAPC-IG||0.5mg vial|
Listing of Protein C Antibodies
GAPC-IG – Goat anti human Protein C, purified IgG (5mg vial)
SAPC-IG – Sheep anti human Protein C, purified IgG (10mg vial)
SAPC-HRP – Sheep anti human Protein C, peroxidase conjugated IgG (0.2 mg vial)
MAPC-IG – Mouse anti human Protein C, monclonal antibody (0.5mg vial)
SAPC-AP – Sheep anti human Protein C, affinity purified IgG (0.5 mg vial)
GAPC-AP – Goat anti human Protein C, affinity purified IgG (0.5 mg vial)
PC-EIA – Protein C Paired Antibody Set – 5 plate set
APCPCI-EIA – APC-Protein C Inhibitor Complex Paired Antibody Set – 5 plate set
Description of Protein C
Protein C (PC) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of PC in plasma is ~4 μg/ml (~60 nM). A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis. Protein C is expressed as a two-chain molecule with a molecular weight of 62 kDa. The light chain (21 kDa) of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11 γ-carboxyglutamic acid (gla) residues. These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The heavy chain of PC (41 kDa) consists of the catalytic domain and an activation peptide. Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin. The activation of PC is associated with the release of a small activation peptide (2-3 kDa, called Protein C peptide, or PCP) from the N-terminal of the heavy chain. Activated Protein C (APC) is a serine protease with anticoagulant activity. APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa. The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, also called Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by α1antitrypsin and α2macroglobulin. The inhibitory activity of PCI is stimulated approximately 10 fold by heparin1-3.
References and Reviews
- Broze GJ, Miletich JP; Biochemistry and Physiology of Protein C, Protein S and Thrombomodulin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 259-276, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
- Esmon CT, Esmon NL, Le Bonniec B, Johnson AE; Protein C Activation; Methods in Enzymology 222, pp 359-385, 1993.
- Heeb MJ, Mosher D, Griffin JH; Activation and Complexation of Protein C and Cleavage and Decrease of Protein S in Plasma of Patients With Intravascular Coagulation; Blood 73, pp 455-461, 1989.