Affinity Biologicals, Inc. manufactures a broad range of Vimentin antibodies which can be found in the listing below. Further information about each individual Vimentin antibody is available by following the associated links. Our Vimentin antibodies are excellent for us in immunoassays where high sensitivity is required and are frequently the preferred reagent for immunopreciptaion techniques and activity neutralization assays. These antibodies have proven to be extremely useful in the preparation of immune-adsorbent resins for use in immuno-depletion of specific proteins from plasma as well as immuno-affinity purification of proteins. Affinity’s Vimentin antibodies are manufactured for use in research applications.
Listing of Vimentin Antibodies
SAVM-AP – Sheep anti human Vimentin, affinity purified IgG (0.5 mg vial)
SAVM-APHRP – Sheep anti human Vimentin, affinity purified, peroxidase conj. IgG (0.1 mg vial)
SAVM-IG – Sheep anti human Vimentin, purified IgG (10mg vial)
Description of Vimentin (Vm)
Intermediate filaments (IFs), along with microfilaments and microtubules, are the three major filament systems that form the architectural basis of most eukaryotic cells. Vimentin, with a molecular weight of 58 kDa, is a Type III intermediate filament found in cells of mesenchymal origin including endothelial cells, megakaryocytes and platelets as well as in most cultured cell lines. As with other IFs, vimentin provides a physical linkage between the plasma membrane and the nuclear envelope. The carboxy-terminus of vimentin associates with the lamin B of the nuclear envelope whereas the amino-terminus associates with the plasma membrane either directly or indirectly. In general, it is believed that IFs, including vimentin, provide a network onto which various enzyme systems, intracellular structures and organelles are spatially arranged within the cytoplasm. IFs may also play an active role in the re-organization of intracellular components in response to extracellular signals via their disassembly and reassembly. In normal vascular endothelium, vimentin, along with other cytoskeletal proteins, are typically not exposed to extracellular plasma proteins. However, with damage to endothelial cells, exposure of these cytoskeletal components may play a role in thrombogenesis or inflammatory responses. For example, it has been demonstrated that vimentin can bind complement components as wells as immunoglobulins. Vimetin has also been shown to bind vitronectin and vitronectin/PAI-1 complexes in LPS-damaged endothelial cells.1-3
References and Reviews
- Steinert, P.M. and Roop, D.R. Molecular and cellular biology of intermediate filaments. Ann. Rev. Biochem. 57:593-625, 1988.
- Albrect, D.L., Mills, J.W. and Noelle, R.J. Membrane Ig-Cytoskeletal interatctions: receptor cross-linking results in the formation of extensive filamentous arrays of vimentin. J. Immunol. 144:3251-3256, 1990.
- Podor, T.J. and Loskutoff, D.J. Binding of PAI-1/vitronectin complexes to the intermediate filament cytoskeleton of endotoxin injured endothelial cells. Fibrinolysis (suppl.), 4:263a, 1990.