Factor VIII Deficient Plasma
Factor VIII Deficient Plasma from Affinity Biologicals is manufactured from pooled normal human plasma depleted of FVIII using antibodies directed to FVIII complex. Only the highest quality citrated plasma is used as starting material. Our Deficient Plasma products are typically buffered with the addition of HEPES to 20mM final concentration.
All Factor VIII Deficient Plasma (FVIII-DP) from Affinity Biologicals are for Research Use Only and must not be used for diagnostic applications.
Factor VIII Deficient – Depleted Plasma
Factor VIII Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Factor VIII by selective affinity immuno-adsorption using antibodies directed towards FVIII.
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and are available in 1ml vials to litre quantities. Our Factor VIII deficient – depleted plasma can be used for further manufacturing or research use only applications.
Product Code: FVIII-DP
Presentation: Frozen Factor VIII Deficient – Depleted Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Certificate of Analysis: available upon request
Description of Factor VIII (FVIII)
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320 kDa) that circulates in plasma at approximately 200 ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of FVIII ranging in size from 200-280 kDa. The FVIII is stabilized by association with von Willebrand Factor to form a FVIII-vWF complex required for the normal survival of FVIII in vivo (t1/2 of 8-12 hours).
F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, FVIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay.
Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of FVIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a FVIII protein that is partially or totally inactive. The production of neutralizing antibodies to FVIII also occurs in 5-20% of Hemophiliacs 1-3.
References and Reviews
- Lollar P, Fay PJ, Fass DN; Factor VIII and Factor VIIIa. Methods in Enzymology, 222, pg 122, 1993.
- Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
- Pittman DD, Kaufman RJ. Structure-Function Relationships of Factor VIII Elucidated through Recombinant DNA Technology. Thromb. Haemostas. 61:161-165, 1989.
FVIII-DP – Factor VIII Deficient Plasma – 1ml or bulk quantities
SAC8C-HRP – Sheep anti-canine Factor VIII:C,peroxidase conjugated IgG (0.2 mg vial)
SAF8C-APBIO – Sheep anti human Factor VIII:C, affinity purified, Biotinylated IgG (0.1 mg vial)
SAF8C-AP – Sheep anti human Factor VIII:C, affinity purified IgG (0.5 mg vial)
CFVIII-EIA –Canine Factor VIII Paired Antibody Set – 4 plate set
SAF8C-APFTC – Sheep anti human Factor VIII:C, affinity purified, FITC conjugated IgG (0.1 mg vial)
FVIII-AG – Complete Factor VIII Antigen ELISA Kit – 1 plate/kit
SAF8C-HRP – Sheep anti human Factor VIII:C, peroxidase conjugated IgG (0.2 mg vial)
F8C-EIA – Factor VIII:C Paired Antibody Set – 4 plate set
FRDP080105 – VisuDep-F Frozen Factor VIII deficient plasma – 5 x 1ml Box (IVD)
FRDP080125 – VisuDep-F Frozen Factor VIII deficient plasma – 25 x 1ml Box (IVD)
SAC8C-IG – Sheep anti Canine Factor VIII, purified IgG (5mg vial)
SAF8C-IG – Sheep anti human Factor VIII, purified IgG (10mg vial)
INH8-DP – Frozen Factor VIII Inhibitor Plasma – 1ml and bulk
INH8-LDP – Lyophilized Factor VIII Inhibitor Plasma – 1ml