Factor VIII Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Factor VIII by selective affinity immuno-adsorption using antibodies directed towards FVIII. Our Factor VIII Deficient Plasma is deficient in FVIII to less than 1% activity. During the depletion process vWF levels are reduced to below normal levels.
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and are available in 1ml vials to litre quantities. Our Factor VIII deficient – depleted plasma can be used for further manufacturing or research use only applications.
Product Code:FVIII-DP
Presentation: Frozen Factor VIII Deficient – Depleted Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320 kDa) that circulates in plasma at approximately 200 ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of FVIII ranging in size from 200-280 kDa. The FVIII is stabilized by association with von Willebrand Factor to form a FVIII-vWF complex required for the normal survival of FVIII in vivo (t1/2 of 8-12 hours).
F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, FVIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay.
Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of FVIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a FVIII protein that is partially or totally inactive. The production of neutralizing antibodies to FVIII also occurs in 5-20% of Hemophiliacs 1-3.
References and Reviews
Lollar P, Fay PJ, Fass DN; Factor VIII and Factor VIIIa. Methods in Enzymology, 222, pg 122, 1993.
Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
Pittman DD, Kaufman RJ. Structure-Function Relationships of Factor VIII Elucidated through Recombinant DNA Technology. Thromb. Haemostas. 61:161-165, 1989.
Factor VIII Deficient – Depleted Plasma
FVIII-DP – Factor VIII Deficient Plasma – 1ml or bulk quantities
Description
Factor VIII Deficient – Depleted Plasma
Factor VIII Deficient – Depleted Plasma is manufactured from normal citrated human plasma that has been depleted of Factor VIII by selective affinity immuno-adsorption using antibodies directed towards FVIII. Our Factor VIII Deficient Plasma is deficient in FVIII to less than 1% activity. During the depletion process vWF levels are reduced to below normal levels.
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Plasma products are typically buffered with the addition of HEPES to 20mM final concentration and are available in 1ml vials to litre quantities. Our Factor VIII deficient – depleted plasma can be used for further manufacturing or research use only applications.
Product Code: FVIII-DP
Presentation: Frozen Factor VIII Deficient – Depleted Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Certificate of Analysis: available upon request
Description of Factor VIII (FVIII)
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320 kDa) that circulates in plasma at approximately 200 ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of FVIII ranging in size from 200-280 kDa. The FVIII is stabilized by association with von Willebrand Factor to form a FVIII-vWF complex required for the normal survival of FVIII in vivo (t1/2 of 8-12 hours).
F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, FVIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay.
Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of FVIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a FVIII protein that is partially or totally inactive. The production of neutralizing antibodies to FVIII also occurs in 5-20% of Hemophiliacs 1-3.
References and Reviews
Related Products:
Canine Factor IX Paired Antibody Set
CFIX-EIA – Canine Factor IX Paired Antibody Set – 4 plate set
Factor IX Polyclonal Antibody – Affinity Purified – FITC Conjugated
GAFIX-APFTC – Goat anti human Factor IX, affinity purified, FITC conjugated IgG (0.1 mg vial)
Hemostasis Control Plasma – Normal
LCSN01 – VisuCon-L Coag Screen N – 10 x 1ml box (IVD)
Factor X Polyclonal Antibody – HRP Conjugated – Rabbit
RAFX-HRP – Rabbit anti human Factor X, peroxidase conjugated IgG (0.2 mg vial)
Plasminogen Polyclonal Antibody – Affinity Purified – Goat
GAPG-AP – Goat anti human Plasminogen, affinity purified IgG (0.5 mg vial)
Factor VIII Paired Antibody Set
F8C-EIA – Factor VIII:C Paired Antibody Set – 4 plate set
Factor VIII:C Polyclonal Antibody – Affinity Purified
SAF8C-AP – Sheep anti human Factor VIII:C, affinity purified IgG (0.5 mg vial)
alpha-2-Antiplasmin Polyclonal Antibody
GA2AP-IG – Goat anti human alpha-2-Antiplasmin, purified IgG (5mg vial)
tPA and PAI-1 Deficient – Depleted Plasma
TPA/PAI-DP – Tissue Plasminogen Activator (tPA) and Plasminogen Activator Inhibitor – Type 1 (PAI-1) Deficient Plasma – 1ml or bulk quantities
TAFI Antigen ELISA Kit
TAFI-AG – Complete TAFI Antigen ELISA Kit – 1 plate/kit
PCI – Protein C Inhibitor Polyclonal Antibody – Affinity Purified
GAPCI-AP – Goat anti human PCI – Protein C Inhibitor , affinity purified IgG (0.5 mg vial)
Routine Normal Control Plasma
FCSN0125 – VisuCon-F Frozen Coag Screen N – 25 x 1ml box (IVD)
ATIII – Antithrombin Polyclonal Antibody – Affinity Purified – Sheep
SAAT-AP – Sheep anti human ATIII – Antithrombin, affinity purified IgG (0.5 mg vial)
Prothrombin – Factor II Polyclonal Antibody – Affinity Purified
SAFII-AP – Sheep anti human Prothrombin, affinity purified IgG (0.5 mg vial)
Prothrombin – Factor II Polyclonal Antibody
SAFII-IG – Sheep anti human Prothrombin – Factor II, purified IgG (10mg vial)
TFPI – Tissue Factor Pathway Inhibitor Polyclonal Antibody
SATFPI-IG – Sheep anti human TFPI, purified IgG (10mg vial)
Protein S Polyclonal Antibody – Affinity Purified
SAPS-AP – Sheep anti human Protein S, affinity purified IgG (0.5 mg vial)
Factor V Polyclonal Antibody
SAFV-IG – Sheep anti human Factor V, purified IgG (10mg vial)
Factor VIII:C Polyclonal Antibody – Affinity Purified – FITC Conjugated
SAF8C-APFTC – Sheep anti human Factor VIII:C, affinity purified, FITC conjugated IgG (0.1 mg vial)
Non-Immune Rabbit IgG
NIR-IG – Non-Immune Rabbit Immunoglobulin, purified IgG (10mg vial)
Factor XI Deficient – Depleted Plasma
FXI-DP – Factor XI Deficient Plasma – 1ml or bulk quantities
ATIII – Antithrombin Polyclonal Antibody – Affinity Purified – Goat
GAAT-AP – Goat anti human ATIII – Antithrombin, affinity purified IgG (0.5 mg vial)
Factor X Deficient – Depleted Plasma
FX-DP – Factor X Deficient Plasma – 1ml or bulk quantities
Factor XIII Deficient – Depleted Plasma
FXIII-DP – Frozen Factor XIII Deficient Plasma – 1ml or bulk quantities
FXIII-LDP – Lyophilized Factor XIII Deficient Plasma – 1ml
Factor IX Polyclonal Antibody – Affinity Purified – HRP Conjugated – Sheep
SAFIX-APHRP – Sheep anti human Factor IX, affinity purified, peroxidase conj. IgG (0.1 mg vial)
TAFI Deficient – Depleted Plasma
TAFI-DP – Frozen TAFI Deficient Plasma – 1ml or bulk quantities
TAFI-LDP – Lyophilized TAFI Deficient Plasma – 1ml
Factor V Polyclonal Antibody – Affinity Purified
SAFV-AP – Sheep anti human Factor V, affinity purified IgG (0.5 mg vial)
Non-immune Goat Antibody
NIG-IG – Non-Immune Goat Immunoglobulin, purified IgG (10mg vial)
Prothrombin Fragment – 2 Polyclonal Antibody – Affinity Purified
SAFII-F2AP – Sheep anti human Prothrombin Fragment – 2, affinity purified IgG (0.5 mg vial)
Factor V Paired Antibody Set
FV-EIA – Factor V Paired Antibody Set – 5 plate set
Protein S Polyclonal Antibody – HRP Conjugated – Sheep
SAPS-HRP – Sheep anti human Protein S, peroxidase conjugated IgG (0.2 mg vial)
Protein C Polyclonal Antibody – Affinity Purified – Goat
GAPC-AP – Goat anti human Protein C, affinity purified IgG (0.5 mg vial)
Factor XI Inhibitor Plasma
INH11-DP – Frozen Factor XI Inhibitor Plasma – 1ml and bulk
vWF – von Willebrand Factor Polyclonal Antibody – HRP Conjugated
GAVWF-HRP – Goat anti human vWF – von Willebrand Factor, peroxidase conjugated IgG (0.15 mg vial)
Fibrinopeptide A Polyclonal Antibody
SAFPA-IG – Sheep anti human Fibrinopeptide A, purified IgG (10mg vial)
Factor IX Polyclonal Antibody – HRP Conjugated
GAFIX-HRP – Goat anti human Factor IX, peroxidase conjugated IgG (0.2 mg vial)
Prekallikrein Deficient – Depleted Plasma
PK-DP – Frozen Prekallikrein Deficient Plasma – 1ml or bulk quantities
PK-LDP – Lyophilized Prekallikrein Deficient Plasma – 1ml
Plasminogen Deficient – Depleted Plasma
PG-DP – Frozen Plasminogen Deficient Plasma – 1ml or bulk quantities
PG-LDP – Lyophilized Plasminogen Deficient Plasma – 1ml
Hemostasis Control Plasma (High-Level Abnormal)
LCSAB201 – VisuCon-L Coag Screen ABN2 – 10 x 1ml Box (IVD)
Factor XIII (A-Subunit) Polyclonal Antibody
SAF13A-IG – Sheep anti human Factor XIII (A-subunit), purified IgG (10mg vial)