Back to "ELISA Kits"

Factor VIII Antigen Kit

ELISA Kits

The VisuLize™ Factor VIII Antigen Kit is intended to quantitatively measure Factor VIII (FVIII) antigen in human plasma and factor concentrates. The biological importance of FVIII is demonstrated in hemophilia A and those patients suspected of thrombophilia. The laboratory diagnosis of FVIII deficiency in hemophilia A typically involves quantitative determination of FVIII’s functional activity. The use of this kit in conjunction with functional assays in the area of gene therapy, assessment of FVIII concentrates, as well as distinguishing those patients with cross-reactive material will prove useful in the area of hemophilia A. Additionally as documented in numerous studies, elevated levels of FVIII is being recognized as a common risk of venous thrombosis¹, an area where Affinity’s Factor VIII ELISA Kit has utility.

Features

  • Rapid sandwich ELISA to measure FVIII antigen (FVIII:Ag)
  • 1 hour 55 minutes total incubation time
  • FVIII:Ag reported as International Units/mL traceable to WHO standard for FVIII antigen
  • Detection limit to 0.008 IU/mL FVIII:Ag (0.8%)
  • Includes normal and low controls

Applications

  • Quantification of FVIII antigen in plasma and therapeutic concentrates
  • Diagnosis and characterization of hemophilia A
  • Carrier testing for hemophilia A
  • Thrombophilia screening

¹O’Donnell J, Tuddenham EG, Manning R, Kemball-Cook G, Johnson D, Laffan M. High prevalence of elevated factor VIII levels in patients referred for thrombophilia screening: role of increased synthesis and relationship to the acute phase reaction. Thromb Haemost. 1997;77:825–828.

View Species Cross Reactivity Chart

Specifications

Storage and Shelf Life

Storing

between 2°C and 8°C

Type

Liquid

Type

Lyophilized

Expiration

Please inquire

Available Formats

Cat # Format
FVIII-AG

Pre-coated 96-well plate; 6x16 strip format

2 x Calibrator Plasma

2 x Control Plasma A

2 x Control Plasma B

1 x 50.0 mL 20X Wash Buffer Concentrate

3 x 20.0 mL Buffered Sample Diluent

1 x 12.0 mL Detecting Antibody

1 x 12.0 mL TMB Substrate

1 x 12.0 mL Stop Solution

US and Canada. Available For Research Use Only elsewhere (catalog number FVIII-AG-RUO).
Interested?
Get a Quote

Ask Us

Our knowledgeable staff are here for you. If you have any technical questions about our products or techniques, don't hesitate to call or email us.