TAFI Antigen ELISA Kit

TAFI Antigen ELISA Kit

The VisuLize TAFI Antigen ELISA Kit is an Enzyme Immunoassay for the quantitative determination of TAFI Antigen in human plasma samples using the double antibody enzyme linked immuno-sorbent assay (ELISA).  This TAFI Antigen ELISA Kit is for Research Use Only and must not be used for diagnostic applications.

Product Code: TAFI-AG

Features:

  • Rapid sandwich ELISA to measure TAFI antigen in plasma
  • 90 minutes total incubation time!
  • TAFI values reported as ug/ml or nanomolar (nM)
  • TAFI measurements traceable to purified TAFI based on Extinction (1%280) = 22.0
  • Reduced sensitivity to activated TAFI (TAFIa)
  • Insensitive to therapeutic levels of heparin or EACA

Kit Contents:

  • Pre-coated plate in a convenient 96 well; 6×16 strip format
  • Calibrator plasma, Control A and Control B plasmas (2 vials of each)
  • Sample diluent and wash buffer concentrate
  • Ready-to-use detecting antibody, TMB substrate and stop solutions

Species Cross Reactivity: View Chart

Product Datasheet: VisuLize-TAFI-Antigen-complete-ELISA-KIT-Affinity-Biologicals


Description of TAFI

TAFI (Thrombin Activatable Fibrinolysis Inhibitor), also referred to as plasma procarboxypeptidase-B, procarboxypeptidase-U and R, circulates in plasma as a zymogen with a mass of 58,000 daltons. Proteolytic activation of TAFI yields an N-terminally derived activation peptide and the C-terminal portion corresponding to the metalloprotease, activated TAFI (TAFIa). TAFIa exhibits exopeptidase activity with carboxypeptidase B-like substrate specificity capable of catalyzing the hydrolysis of C-terminal lysine and arginine residues. Cleavage of these residues on fibrin by TAFIa attenuates clot lysis by inhibiting the formation of the ternary activation complex comprising fibrin cofactor, tPA and plasminogen, thereby inhibiting plasmin generation. Although TAFI can be activated by various proteases including thrombin and plasmin, the physiological activator is proposed to be the complex thrombin-thrombomodulin since the rate of activation is stimulated 1250-fold compared to thrombin alone¹. However, the rate of TAFI activation is highly dependent upon its plasma concentration. Since TAFIa apparently plays a key role in connecting coagulation and fibrinolysis and significantly increases clot stability, determination of plasma concentration of TAFI is likely crucial to assess its subsequent potential anti-fibrinolytic effects. This has been demonstrated in a report identifying high plasma concentration of TAFI as a risk factor for thrombosis².

References and Reviews

Bajzar, L., Morser, J., Nesheim, M.E. TAFI, or procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex. J.Biol.Chem. 271:16603-16608, 1996.

Van Tilburg, N.H., Rosendaal, F.R., Bertina R.M., Thrombin Activatable fibrinolysis inhibitor and the risk for deep vein thrombosis. Blood. 95:2855-2859, 2000.

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Description of TAFI

TAFI (Thrombin Activatable Fibrinolysis Inhibitor), also referred to as plasma procarboxypeptidase-B, procarboxypeptidase-U and R, circulates in plasma as a zymogen with a mass of 58,000 daltons. Proteolytic activation of TAFI yields an N-terminally derived activation peptide and the C-terminal portion corresponding to the metalloprotease, activated TAFI (TAFIa). TAFIa exhibits exopeptidase activity with carboxypeptidase B-like substrate specificity capable of catalyzing the hydrolysis of C-terminal lysine and arginine residues. Cleavage of these residues on fibrin by TAFIa attenuates clot lysis by inhibiting the formation of the ternary activation complex comprising fibrin cofactor, tPA and plasminogen, thereby inhibiting plasmin generation. Although TAFI can be activated by various proteases including thrombin and plasmin, the physiological activator is proposed to be the complex thrombin-thrombomodulin since the rate of activation is stimulated 1250-fold compared to thrombin alone¹. However, the rate of TAFI activation is highly dependent upon its plasma concentration. Since TAFIa apparently plays a key role in connecting coagulation and fibrinolysis and significantly increases clot stability, determination of plasma concentration of TAFI is likely crucial to assess its subsequent potential anti-fibrinolytic effects. This has been demonstrated in a report identifying high plasma concentration of TAFI as a risk factor for thrombosis².

References and Reviews

Bajzar, L., Morser, J., Nesheim, M.E. TAFI, or procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex. J.Biol.Chem. 271:16603-16608, 1996.

Van Tilburg, N.H., Rosendaal, F.R., Bertina R.M., Thrombin Activatable fibrinolysis inhibitor and the risk for deep vein thrombosis. Blood. 95:2855-2859, 2000.

Related Products: