Antibodies to Thrombin

Thrombin, human

Antibody Host Catalogue No. Size Product Insert
Purified IgG Sheep SAHT-IG 10 mg Thrombin - human - polyclonal antibody - sheep - purified whole IgG
Affinity purified IgG Sheep SAHT-AP 0.5 mg Thrombin - human - polyclonal antibody - sheep - affinity purified IgG
Peroxidase conjugated IgG Sheep SAHT-HRP 0.2 mg Thrombin - human - polyclonal antibody - sheep - peroxidase conjugated IgG
Affinity purified, FITC conjugated IgG Sheep SAHT-APFTC 0.1 mg Thrombin - human - polyclonal antibody - affinity purified FITC conjugated IgG
Affinity purified, biotinylated IgG Sheep SAHT-APBIO 0.1 mg Thrombin - human - polyclonal antibody - sheep - affinity purified biotinylated conjugated IgG
Matched Pair Antibodies
– For EIA of Thrombin-Antithrombin Complex TAT-EIA 5 plates Thrombin-Antithrombin Complex (TAT) - matched-pair antibodies for ELISA
– For EIA of Thrombin-HCII Complex THCII-EIA 5 plates Thrombin-Heparin Cofactor II complex (THCII) - matched-pair antibodies for ELISA

Thrombin, bovine

Antibody Host Catalogue No. Size Product Insert
Purified IgG Sheep SABT-IG 10 mg Thrombin - bovine - polyclonal antibody - sheep - purified whole IgG
Affinity purified IgG Sheep SABT-AP 0.5 mg Thrombin - bovine - polyclonal antibody - sheep - affinity purified IgG
Peroxidase conjugated IgG Sheep SABT-HRP 0.2 mg

Thrombin, rabbit

Antibody Host Catalogue No. Size Product Insert
Purified IgG Sheep SART-IG 10 mg
Affinity purified IgG Sheep SART-AP 0.5 mg Thrombin - Rabbit - polyclonal antibody - sheep - affinity purified IgG
Peroxidase conjugated IgG Sheep SART-HRP 0.2 mg Thrombin - Rabbit - polyclonal antibody - sheep - peroxidase conjugated IgG

Description of Thrombin

Thrombin (EC3.4.21.5, α-thrombin) is the product of proteolytic activation of the zymogen prothrombin.  Human thrombin is a two-chain serine protease with a mass of 37 kDa.  The active site is located within the heavy chain.  Thrombin has a high specificity for certain arginine bonds in protein substrates.  The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds.  Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B).  In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V.  The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V.  The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin’s specificity, increasing it’s activity toward Protein C and TAFI, and decreasing it’s activity toward fibrinogen and activating cofactors VIII and V.  In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II.  The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin.  Other physiological inhibitors of thrombin in the absence of heparin include α2-macroglobulin and α1-antitrypsin1-4.

References and Reviews
  1. Weitz J, Hudoba M, Massel D, Maranganore J, Hirsch J: Clot-bound
  2. Mann KG; Prothrombin and Thrombin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 184-199, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
  3. Stubbs MT, Bode W; A Player of Many Parts: The Spotlight Falls on Thrombin’s Structure; Thrombosis Research 69, pp 1-58, 1993.
  4. Downing MW, Bloom JW, Mann KG; Comparison of the Inhibition of Thrombin by Three Plasma Protease Inhibitors; Biochemistry 17, pp 2649-2653, 1978.